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Synergistic antibacterial activity of mycosynthesized AgNPs with antibiotics against multidrug resistant Pseudomonas aeruginosa and investigation of protein profile

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Title Synergistic antibacterial activity of mycosynthesized AgNPs with antibiotics against multidrug resistant Pseudomonas aeruginosa and investigation of protein profile
 
Creator Dastidar, Madhumita Ghosh
Nallal, V. Uma Maheshwari
Razia, M
 
Subject Antibacterial activity
Protein expression
Silver nanoparticles
Synergistic activity
 
Description 842-848
Silver nanoparticles (AgNPs) have been identified as promising bactericidal agents especially towards multidrug
resistant bacteria (MDR). Fungi are excellent biogenic sources for synthesizing AgNPs with antibacterial activity. In the
present study, AgNPs were synthesized using Aspergillus niger extract and characterized. Individual and synergistic
antibacterial activities of AgNPs were tested against isolated hospital strain of MDR Pseudomonas aeruginosa by disc
diffusion assay. SDS-PAGE analysis was performed to analyse the protein expression before and after treatment with
AgNPs. Results revealed the successful synthesis of AgNPs using A. nigerby 24 hrs and the average particle size was
measured as 18.72±0.2 nm. AgNPs were predominantly spherical and monodispersed. 18±0.05 mm zone of inhibition was
observed for P. aeruginosa when treated with AgNPs alone whereas synergistic treatment of synthesized AgNPs along with
Tetracycline showed impressive antibacterial activity (27.32±2 mm). Protein expression of P. aeruginosa varied before and
after treatment which was evident from the results. This indicated the effective breakdown of cell wall and cellular proteins
on the synergistic treatment of AgNPs and antibiotics. Thus the study demonstrates a feasible and eco-friendly approach to
synthesize AgNPs with enhanced antibacterial activity against MDR bacterial strains.
 
Date 2021-12-02T06:50:31Z
2021-12-02T06:50:31Z
2021-12
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/58572
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.59(12) [December 2021]