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Promising antimicrobial protein from Klebsiella

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Title Promising antimicrobial protein from Klebsiella
 
Creator Shahadani, Ashwini A.
M, Rajyalakshmi
Hadagali, Ashoka
Vinjamuri, Saisha
 
Subject Antimicrobial peptides
APD3 software
Bacteriocins
CAMPR3 software
 
Description 834-841
Increasing antibody resistance necessitates the use of new antimicrobial products. Antimicrobial peptides (AMP`s) And
Proteins are a promising alternative to antibiotics currently being used due to their high degree of specificity & wide range
of action Here we report a novel antimicrobial protein from iron reducing bacteria isolated from the coffee estate soils of
Coorg district, Karnataka, India. Iron reducing bacteria are known to be antagonistic to others due to the presence of
Pseuderophores. It makes them a good source of antimicrobial active principles. Fifteen isolates were obtained by
selectively screening for iron reduction. These isolates were tested for antimicrobial activity against Gram positive
[Staphylococcus aureus (MTCC3160), Bacillus cereus (MTCC6620)] & Gram negative [Escherichia coli (MTCC1650),
Salmonella typhi (MTCC3224) and Pseudomonas aeruginosa (MTCC2453)] bacteria. Of the 15 isolates, one isolate, CBI-8,
which showed good antimicrobial activity, was selected for further studies. The isolate was identified by MALDI-TOF &
16S rDNA sequencing as Klebsiella variicola/quasipneumonae (strain IS93) (genebank - MN814029). To induce and
augment the production of antimicrobial molecules, CBI-8 was grown in the presence of Salmonella typhi and the spent
medium was subjected to ammonium sulphate precipitation. The fractions which showed promising activity were further
subjected to preparatory HPLC. The distinct bands obtained in the SDS-PAGE corresponding to the HPLC peak that showed
antimicrobial activity from the induced sample was analyzed by Mass Spectrometry. Antimicrobial Peptide Database
(APD3) calculator, Collection of Anti-Microbial Peptides (CAMPR3) software used to predict AMPs in the proteins
identified from MS data.
 
Date 2021-12-02T06:54:12Z
2021-12-02T06:54:12Z
2021-12
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/58573
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.59(12) [December 2021]