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Low temperature co-fired ceramic (LTCC)- based biosensor for detection of vanadium using immobilized Arachis hypogaea alkaline phosphatase on multi walled carbon nanotubes ethyl cellulose sponge matrix

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Title Statement Low temperature co-fired ceramic (LTCC)- based biosensor for detection of vanadium using immobilized Arachis hypogaea alkaline phosphatase on multi walled carbon nanotubes ethyl cellulose sponge matrix
 
Added Entry - Uncontrolled Name Giramkar, Vijaya D; Centre for Materials for Electronics Technology, Panchwati, Off Pashan road, Pune-411 008, Maharashtra, India
Phatak, Girish J
Sabharwal, Sushma G
 
Uncontrolled Index Term Alkaline phosphatase; Electronic packaging; Heavy metal toxicity; MWCNTs; Thermometric sensor
 
Summary, etc. Studies on enzyme based thermometric biosensor are limited. Here, we report on fabrication of an alkaline phosphate based thermometric biosensor. We designed alkaline phosphatase inhibition based biosensor for detection of vanadium using immobilized alkaline phosphatase on multi walled carbon nano tubes (MWCTs) ethyl cellulose sponge matrix. We isolated protein from plant source, partially purified and fabricated a miniature ceramic viz. LTCC (low temperature co-fired ceramics technology) based biosensor for detection of vanadium. This biosensor consists of a microreaction chamber with buried heaters. Alkaline phosphatase has been isolated from the seeds of ‘Arachis hypoghaea’ was studied for its biochemical properties viz. optimum pH and temperature. The partially purified enzyme was immobilized using carboxyl-functionalised carbon nanotubes (CNTs) by cross linking with epichlorohydrin (ECH) along with a matrix of ethyl cellulose. The developed LTCC based biosensor on testing indicated its linear response to vanadium concentration up to 9 mM with a relatively high sensitivity of about 147 nA/mM. Thus, we have demonstrated a LTCC based biosensor using immobilized alkaline phosphatase for detection of vanadium.
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2021-12-02 00:00:00
 
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http://op.niscair.res.in/index.php/IJEB/article/view/57842
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 59, ##issue.no## 12 (2021): IJEB [DECEMBER 2021]
 
Language Note en