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Antioxidant efficacy and cytotoxicity of ethanol extract of Clerodendrum infortunatum against different cell lines

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Title Antioxidant efficacy and cytotoxicity of ethanol extract of Clerodendrum infortunatum against different cell lines
 
Creator Malsawmdawngliana
Zohmachhuana, Alex
Vabeiryureilai, M
Thangjam, Nurpen Meitei
Lalrinzuali, K
Kumar, N Senthil
Kumar, Awadhesh
 
Subject Antioxidant
Cell lines
Clerodendrum infortunatum
GC-MS, Mizoram
 
Description 572-581
Clerodendrum infortunatum belongs to the Lamiaceae family and is a perennial shrub. It is widely known for their
important medicinal values among the Mizo tribe. In the present study, the preliminary phytochemical screening,
quantification of phenols, flavonoids and alkaloids, antioxidant activities by DPPH, O2- and ABTS assays and cytotoxicityby
MTT assay against AGS (gastric cancer), HeLa (cervical) and HT-29 (colon) cell lines compared with normal cell line
(Chang liver) were performed. Furthermore, the GC-MS profiling was also conducted. The results imply the presence of
saponin, alkaloid, cardiac glycoside, phenol and flavonoid. The quantification shows that phenol content (64.35 mg/ g) was
highest followed by flavonoid (61.93 mg/ g) and alkaloid (13.33 mg/ g). Its scavenging efficiency against DPPH with IC50
value was 47.99, against O2- with IC50 was108 µg/mL and against ABTS cations with IC50 was 50.05 µg/mL, respectively.
The ethanol extract exhibited a maximum cytotoxicity against HeLa with IC50 value of 53.55 µg/mL, AGS with IC50 value
82.44 µg/mL and HT-29 with IC50 value of 142.2 µg/mL. However, the extract showed comparatively less toxicity against
normal cell lines. Moreover, 14 active compounds were confirmed in the GC-MS analysis of the extract. HPLC study also
infers the occurrence of the flavonoids rutin and quercetin. Therefore, the results of C. infortunatum ethanolic extract clearly
specified that it has a very high antioxidant activity as well as cytotoxic properties; which proved that this ethnomedicinal
plant can be used as an alternative agent to treat a variety of illnesses.
 
Date 2021-12-07T05:16:47Z
2021-12-07T05:16:47Z
2021-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscair.res.in/handle/123456789/58597
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.58(6) [December 2021]