Record Details

Detection of Salmonella by Surface Plasmon Resonance

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/11948/
https://doi.org/10.3390/s7081427
doi:10.3390/s7081427
 
Title Detection of Salmonella by Surface Plasmon Resonance
 
Creator Barlen, B
Mazumdar, S D
Lezrich, O
Kämpfer, P
Keusgen, M
 
Subject Food and Nutrition
 
Description This study explores the possibility of simultaneous and specific detection of
Salmonella serovars by surface plasmon resonance (SPR). The Plasmonic® SPR device was
used to develop this rapid assay. The sandwich immunoassay involves the use of a
polyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovars
present in a sample. This is followed by specific detection of the captured serovars using
O-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium and
Salmonella Enteritidis was used as a model system to establish the assay. The assay was
further extended to sequentially differentiate between the two Salmonella serovars on a
single SPR chip in a single channel. The assay was proved to work without any additional
dilution or clean-up steps. The sample volume requirement for the assay is only 10 μL. The
lower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were
2.50×105 cells mL-1 and 2.50×108 cells mL-1, respectively.
 
Publisher MDPI
 
Date 2007-08
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11948/1/sensors-07-01427.pdf
Barlen, B and Mazumdar, S D and Lezrich, O and Kämpfer, P and Keusgen, M (2007) Detection of Salmonella by Surface Plasmon Resonance. Sensors, 7 (8). pp. 1427-1446. ISSN 1424-8220