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Molecular phylogeny of commercially important lobster species from Indian coast inferred from mitochondrial and nuclear DNA sequences

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Relation http://eprints.cmfri.org.in/10401/
http://informahealthcare.com/mdn
 
Title Molecular phylogeny of commercially important lobster species from
Indian coast inferred from mitochondrial and nuclear DNA sequences
 
Creator Jeena, N S
Gopalakrishnan, A
Radhakrishnan, E V
Kizhakudan, Joe K
Basheer, V S
Asokan, P K
Jena, J K
 
Subject Fish Biotechnology
Lobsters
 
Description Lobsters constitute low-volume high-value crustacean fishery resource along Indian coast.
For the conservation and management of this declining resource, accurate identification of
species and larvae is essential. The objectives of this work were to generate species-specific
molecular signatures of 11 commercially important species of lobsters of families Palinuridae
and Scyllaridae and to reconstruct a phylogeny to clarify the evolutionary relationships among
genera and species included in this study. Partial sequences were generated for all the
candidate species from sampling sites along the Indian coast using markers like Cytochrome
oxidase I (COI), 16SrRNA, 12SrRNA, and 18SrRNA genes, and analyzed. The genetic identities of
widely distributed Thenus species along the Indian coast to be Thenus unimaculatus and the
sub-species of Panulirus homarus to be P. homarus homarus were confirmed. Phylogeny
reconstruction using the individual gene and concatenated mtDNA data set were carried out.
The overall results suggested independent monophyly of Scyllaridae and Stridentes of
Palinuridae. The interspecific divergence was found to be highest for the 12SrRNA compared
with other genes. Significant incongruence between mtDNA and nuclear 18SrRNA gene tree
topologies was observed. The results hinted an earlier origin for Palinuridae compared with
Scyllaridae. The DNA sequence data generated from this study will aid in the correct
identification of lobster larvae and will find application in research related to larval transport
and distribution.
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/10401/1/Jeena_Lobster_Mitochondrial_DNA.pdf
Jeena, N S and Gopalakrishnan, A and Radhakrishnan, E V and Kizhakudan, Joe K and Basheer, V S and Asokan, P K and Jena, J K (2015) Molecular phylogeny of commercially important lobster species from Indian coast inferred from mitochondrial and nuclear DNA sequences. Mitochondrial DNA. pp. 1-10.