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Development of transgenic cucumber mosaic virus (CMV) resistant gerbera plants expressing CMV coat protein gene

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Title Development of transgenic cucumber mosaic virus (CMV) resistant gerbera plants expressing CMV coat protein gene
 
Creator Gautam, KK
Raj, Rashmi
Kumar, Susheel
Agrawal, Lalit
Chauhan, PS
Raj, SK
 
Subject Challenge inoculations
Floriculture industry
Ornamental crops
RNA virus
Virus resistance
 
Description 121-130
Gerbera (Gerbera jamesonii L.) has its immense importance to the floriculture industry worldwide. The gerbera flower
production has been hampered by various viruses, among them cucumber mosaic virus (CMV) has shown considerable
damage.As natural resistance to CMV is absent in gerbera, here, we have made an attempt to develop transgenic gerbera
plants expressing coat protein (CP) gene of CMV via Agrobacterium mediated transformation of base petiole explants for
genetic resistance to CMV infection. Among the 44 putative transgenic gerbera plant acclimatized, 39 were found positive
for integration of CP gene by polymerase chain reaction and southern hybridization assay using their specific primer and
probe respectively. Northern hybridization assay using CP gene specific probe confirmed the transcription of transgene in all
39 transgenic plants. These plants showed translation of CP during DAS-ELISA when tested with antiserum specific to CP
of CMV. These 39 plants when challenged by mechanical inoculations with CMV gerbera isolate showed virus resistance in
53% (21 out of 39) plants, virus tolerance (delayed mild symptom) in 33% (13/39) plants, while rest 12.8% (5/39) plants
showed severe disease symptoms. The CP mediated resistance of CMV in transgenic gerbera is being reported for the first
time from India.
 
Date 2022-01-31T10:30:18Z
2022-01-31T10:30:18Z
2022-02
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/59004
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.60(02) [Feb 2022]