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Synthesis of photoactivable Pt(IV) prodrug loaded on NaYF4 based upconversion nanoparticles functionalized with 2-deoxy-D-glucose and its evaluation for targeted cancer therapy

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Title Synthesis of photoactivable Pt(IV) prodrug loaded on NaYF4 based upconversion nanoparticles functionalized with 2-deoxy-D-glucose and its evaluation for targeted cancer therapy
 
Creator Sharma, K Shitaljit
Tiewsoh, Lodestarborn
Dubey, Akhil K
Phadnis, Prasad P
Sudarsan, V
Vatsa, Rajesh K
 
Subject Photoactivable
Pt(IV) prodrug
Upconversion nanoparticles
2-deoxy-D-glucose
Internalization
Clonogenic assay
 
Description 21-30
Nano-formulation based on Tm, Yb doped NaYF4 upconversion nanoparticles (UCNPs) functionalized with 2-deoxy-D-glucose have been synthesized to load the photoactivable Pt(IV) prodrug, cis-[PtI2(NH3)2(OCOCH2CH2COOH)2]. The Pt(IV) prodrug has been synthesized by oxidation of cis-[PtI2(NH3)2] to [PtI2(OH)2(NH3)2] and its further treatment with succinic anhydride. It is loaded through ester bond formation between the carboxyl groups of Pt(IV) prodrug with hydroxyl groups of 2-deoxy-D-glucose (2-DG) coated on UCNPs. The cytotoxicity of formulation after exposing to 385 nm UV light and in absence of light is evaluated against MCF-7 cell lines by MTT assay. The results have revealed enhanced cytotoxicity of UV exposed nano-formulation. Additionally, the clonogenic assay has exhibited the decrease in plating efficiency as inferred from decreased surviving fraction around 20% only for UV activated formulation as compared to formulation in dark, as well as merely Pt(IV) prodrug. These results are indicative that more internalization of the formulation inside the cancer cells was achieved due to the presence of 2-DG rendering more efficiency to kill cancer cells.
 
Date 2022-02-01T11:49:41Z
2022-02-01T11:49:41Z
2022-01
 
Type Article
 
Identifier 2583-1321 (Online); 0019-5103 (Print)
http://nopr.niscair.res.in/handle/123456789/59043
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJC Vol.61(01) [January 2022]