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Incidence and Molecular Typing of Vibrio parahaemolyticus from Tiger Shrimp Culture Environments along the Southwest Coast of India

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Relation http://eprints.cmfri.org.in/8698/
 
Title Incidence and Molecular Typing of Vibrio
parahaemolyticus from Tiger Shrimp Culture Environments
along the Southwest Coast of India
 
Creator Chakraborty, Rekha D
Surendran, P K
 
Subject Fish Biotechnology
Aquaculture
Crustacean Fisheries
Fish Diseases
Fish Pathology
 
Description Vibrio parahaemolyticus is one of the most prevalent food-borne pathogens along the
southwest coast of India, where marine foods are frequently consumed. Shrimp
(Penaeus monodon) and environmental samples were collected from aquaculture farms
located in and around Cochin. Confirmation of the biochemically identified strains with
species-specific toxR gene and detection of virulent genes viz., tdh and trh was
performed by polymerase chain reaction (PCR). The phenotypic markers for the
presence of tdh and trh genes were assayed by Kanagawa phenomenon and urease
activity, respectively. Protease activity was examined to identify other potential
virulence factors. After phenotypic characterization of bacterial strains fingerprinting
of genomic DNA was carried by various typing methods, viz., random amplified
polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus sequence
(ERIC), repetitive extragenic palindromic sequence (REP), and ribosomal gene spacer
sequence (RS) PCR methods to assess the genetic diversity within the isolates.
Eighteen percent of the samples were found positive for the incidence of V. parahaemolyticus
by biochemical protocols and toxR (368 bp) targeted PCR. PCR analyses
revealed 1% of the samples positive for tdh (269 bp) and trh (500 bp) gene. RAPD
analysis revealed clustering of toxigenic strains into a single group. Cluster analysis
revealed the conglomeration of isolates into two, five, and seven major groups using
RS, ERIC, and REP PCR methods, respectively. RS PCR generated fewer amplified
bands compared to REP and ERIC PCR methods, thus giving scope for higher discrimination.
Moreover, RS PCR patterns were more discernible visually from other patterns,
suggesting RS PCR as a considerably practical method for routine use.
 
Publisher Taylor & Francis
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/8698/1/Food_Biotechnology.pdf
Chakraborty, Rekha D and Surendran, P K (2009) Incidence and Molecular Typing of Vibrio parahaemolyticus from Tiger Shrimp Culture Environments along the Southwest Coast of India. Food Biotechnology, 23. pp. 284-311.