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Ocimum sanctum: in vitro antiviral potential against animal viruses

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Title Ocimum sanctum: in vitro antiviral potential against animal viruses
 
Creator Goel, A
Bhatia, A K
 
Subject Antiviral activity
BHV-1
Holy Basil
NDV
Ocimum tenuiflorum
Tulsi
 
Description 120-125
Ocimum sanctum, a well and widely used medicinal plant has been reported to be efficient for many ailments quoted in Ayurvedic literature. It has been reported to treat the various ailments due to viral infections in humans like measles, chickenpox, influenza and Smallpox. Besides, it has also shown its potential to give protection in malignancy related disorders. The present study was carried out to screen its potential against animal viruses infecting the dairy animals. Bovine Herpes Virus-type-1 (BHV-1) and Foot and Mouth disease virus (FMDV) affecting cattles and Newcastle Disease Virus (NDV) affecting poultry were screened. Cytopathic inhibition test was used to check the antiviral effect of O. sanctum against BHV-1 and FMDV in MDBK and BHK cell lines, respectively. Chick embryo fibroblasts were cultured to propagate NDV and tested by haemagglutination inhibition test. The maximum non-toxic dose of Ocimum sanctum leaves was determined in MDBK and BHK cell lines as well as in chick fibroblast by MTT assay. Ocimum sanctum nontoxic concentrations, 2.5 mg/mL, 5 mg/mL and 10 mg/mL were found in MDBK cell line, BHK cell line and chick fibroblast culture respectively. Concentrations lower than MNTD were used for studies. 85.3% and 98.4% protection were recorded against BHV-1 and NDV, respectively. However, no significant effect against FMD virus was observed. Thus, it can be effectively utilised in curing these viral diseases in farm animals.
 
Date 2022-02-15T07:29:35Z
2022-02-15T07:29:35Z
2022-01
 
Type Article
 
Identifier 0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscair.res.in/handle/123456789/59155
 
Language en
 
Relation Int. Cl.22: A61K 36/00, A61K 36/53, A61P 31/12
 
Publisher CSIR-NIScPR
 
Source IJTK Vol.21(1) [January 2022]