Record Details

High resolution-mass spectrometry (HR-MS) analysis of Bryonia laciniosa L.

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title High resolution-mass spectrometry (HR-MS) analysis of Bryonia laciniosa L.
 
Creator M, Shashikala
Shah, Mamta B.
 
Subject Bryonia laciniosa L.
HR-MS analysis
Sterol-rich petroleum ether extract
 
Description 617-624
Bryonia laciniosa L. (Family: Cucurbitaceae) is commonly known as ‘Shivlingi’ and is a highly valued medicinal plant
in Ayurveda and Homeopathy systems of medicine particularly as a remedy for infertility. In the present study,
sophisticated, highly reliable, and sensitive high resolution-mass spectrometry (HR-MS) was carried out for the
determination of chemical constituents present in B. laciniosa. Phytochemical screening of methanolic extract showed the
presence of saponins, sterols, triterpenoids, flavonoids, alkaloids, phenolic compounds, and tannins. TLC of sterol-rich
petroleum ether extract (SRPE) produced six major spots on precoated silica gel GF254, using toluene: methanol (96:04 v/v)
as mobile phase. The spots showed green, purple, light purple to violet colour spots in the Rf range of 0.2-0.91, after
derivatization with the anisaldehyde-sulphuric acid reagent. Further, based on TLC, HR-MS analysis of SRPE was
performed for the identification of phytoconstituents, at positive ESI (electron spray ionisation) mode. It indicated the
presence of a total of 30 compounds including short fragments of peptides. The major compounds predicted in HR-MS:
Q(quadrupole)-TOF(time-of-flight)-MS, as per METLIN database, were swietenine, ergosterol acetate, 4, 4, dimethyl-14α-
formyl-5α-cholesta-8,24-dien-3β-ol, L-olivosyl-oleandolide, mitoxantrone, isogedunin, 3S-aminodeconoic acid, and
nisoldipine.
 
Date 2022-02-15T09:49:09Z
2022-02-15T09:49:09Z
2021-12
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscair.res.in/handle/123456789/59163
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJNPR Vol.12(4) [December 2021]