Phytochemicals enriched Punica granatum L. peel extract promotes the chondrocyte proliferation by inducing cell cycle progression and inhibiting of NO-induced cell death and ROS production
NOPR - NISCAIR Online Periodicals Repository
View Archive InfoField | Value | |
Title |
Phytochemicals enriched Punica granatum L. peel extract promotes the chondrocyte proliferation by inducing cell cycle progression and inhibiting of NO-induced cell death and ROS production
|
|
Creator |
Shivnath, Neelam
Rawat, Vineeta Siddiqui, Sahabjada Jafri, Asif Rais, Juhi Gupta, Madhu Khan, Mohd Sajid Arshad, Md |
|
Subject |
Apoptosis
Chondrocytes Osteoarthritis Oxidative stress Phytochemicals |
|
Description |
17-28
Punica granatum L. has been used as a traditional remedy to treat sore throat, cough, digestive and skin disorders, urinary infection, arthritis and expel tape-worm. Modern research focuses on its use on arthritis, diabetes and cancer. The present study was conducted to identify the bioactive phytochemicals in the ethanolic extract of Punica granatum peel (PGP) through GC-MS to illustrate its proliferation, antioxidant and anti-apoptotic activities in an established in vitro primary cultured chondrocyte. The cells were treated with 25 μM indomethacin, positive control and different concentration of PGP in the absence or presence of different inhibitors such as (H2O2 for ROS production and sodium nitroprusside [SNP] for nitric oxide [NO] induced cell death). The cell viability assay and cell cycle analysis were performed to study the proliferation of cells. Oxidative stress was measured as intracellular ROS and anti-apoptotic activities were studied through nuclear staining assays like DAPI and PI-Exclusion staining. The GC-MS result indicates the presence various antioxidants such as kaempferol, quercetin, myricetin, luteolin, ascorbic acid, gallic acid, vitamin C and lycopene. The PGP treatment on chondrocytes shows increased proliferation and decreased apoptosis through the reduction of oxidative stress in a concentration-dependent manner. The rise in the total number of chondrocytes at higher doses of PGP was significant (p |
|
Date |
2022-02-15T10:10:19Z
2022-02-15T10:10:19Z 2022-01 |
|
Type |
Article
|
|
Identifier |
0975-1068 (Online); 0972-5938 (Print)
http://nopr.niscair.res.in/handle/123456789/59175 |
|
Language |
en
|
|
Relation |
Int. Cl.22: A61K 35/32, A61P 19/02, A61P 19/10
|
|
Publisher |
CSIR-NIScPR
|
|
Source |
IJTK Vol.21(1) [January 2022]
|
|