Record Details

Extraction process optimization of flavonoid and in vitro amylase inhibitory effect of purified quercetin derivative from Amorphophallus paeoniifolius tubers

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Extraction process optimization of flavonoid and in vitro amylase inhibitory effect of purified quercetin derivative from Amorphophallus paeoniifolius tubers
 
Creator R, Balan
P, Indumathi
S, Yuvapriya
V B, Gokhul
P, Muthukumaran
T, Sathishkumar
 
Subject Amorphophallus paeoniifolius
Amylase inhibition
Quercetin derivative
Response surface methodology
 
Description 544-556
Amorphophallus paeoniifolius (Elephant foot yam) is a prominent tuberous plant utilized across several parts of India to
treat various ailments such as a tumour, haemorrhage, microbial infections, cough, bronchitis, diabetes, anaemia, and
hepato-gastro and cardiovascular diseases. In this context, the present study aims to optimize the extraction process of the
flavonoid and to study the in vitro amylase inhibitory effect of purified flavonoid moiety. The Shake flask method with
different extraction solvents was adopted to quantify the flavonoid content. Central composite design (CCD) based response
surface methodology (RSM) was formulated to optimize the extraction process. Three-dimensional preparative
chromatography (3D PTLC) was executed to purify the flavonoid content and high-resolution liquid chromatography-mass
spectrometry (HRLC-MS) was adopted to predict the structure. 3,5-dinitrosalicylic acid (DNS) based spectrophotometry
method was used to determine the amylase inhibitory property. All the analyses were subjected to standard statistical tests.
The developed model for the extraction optimization process was found to be near significant (P = 0.242) with temperature
as a significant variable (P = 0.029), and a 107-fold increase (71.11±0.5 mg/g tissue) of flavonoid content was recorded.
A strong yellow colour spot (flavonoid fraction) was eluted using 3D PTLC technique and the molecule was identified
as quercetin derivative (m/z 447) by the direct MS method. Significant amylase inhibition (36.1±2.1%) recorded by purified
quercetin derivative has documented the utilization of A. paeoniifolius tubers as classical traditional medicine.
 
Date 2022-02-15T10:21:34Z
2022-02-15T10:21:34Z
2021-12
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscair.res.in/handle/123456789/59179
 
Language en
 
Relation Int. cl. (2015.01)- A61K 36/00, A61K 36/888, A61K 125/00, A61P 3/00
 
Publisher NIScPR-CSIR, India
 
Source IJNPR Vol.12(4) [December 2021]