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Advances and trends in analytical techniques in natural product research: Challenges and future perspective

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Title Advances and trends in analytical techniques in natural product research: Challenges and future perspective
 
Creator Verma, Nitin
 
Subject Analytical techniques
Chromatography
Herbal drugs
Natural products
Spectroscopy
 
Description 506-526
Advances in analytical methods and bioassay development have helped to push forward the research in natural products.
Their high chemical diversity and the effects of evolutionary pressure to create biologically active molecules could be
attributed to success in drug discovery. Despite the availability of modern analytical instrumentation techniques, metabolic
profiling covers the identification of a selected group of metabolites. The phytochemical analysis is commonly performed
using standard techniques such as thin-layer chromatography, high-performance thin-layer chromatography, highperformance
liquid chromatography, gas chromatography and more recently mass spectrometry and nuclear magnetic
resonance spectrometry. Two-dimensional J-resolved Nuclear Magnetic Resonance (NMR) spectra and multivariate data
analysis techniques were applied to avoid low resolution and overlapping signals hampering the identification of the
individual components of botanicals. On-targeted metabolomic analysis via the ultra-high performance liquid
chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS) was utilised to localise compounds
belonging to various chemical classes (i.e. oxygenated fatty acids, flavonols, phenolic acids, and sinapoyl choline
derivatives). Rotation planar chromatography over-pressured layer chromatography and electro planar chromatography are
the other innovations. Parallel and serially coupled layers open up new avenues for the analysis of a large number of
samples for high-throughput screening and very complex matrices in a natural product. Analytical strategies with
applications to natural extracts and novel methods that have strong potential, regardless of how often they are used are
discussed with respect to their potential applications challenges and future trends.
 
Date 2022-02-15T10:29:20Z
2022-02-15T10:29:20Z
2021-12
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscair.res.in/handle/123456789/59183
 
Language en
 
Relation Int. cl. (2015.01)- C07B
 
Publisher NIScPR-CSIR, India
 
Source IJNPR Vol.12(4) [December 2021]