Record Details

Effect of poly-D-lysine on proliferation and senescence of caprine spermatogonial stem cells <em>in vitro</em>

Online Publishing @ NISCAIR

View Archive Info
 
 
Field Value
 
Authentication Code dc
 
Title Statement Effect of poly-D-lysine on proliferation and senescence of caprine spermatogonial stem cells <em>in vitro</em>
 
Added Entry - Uncontrolled Name Gupta, Deeksha
Kharche, Suresh Dinkar
Singh, Shiva Pratap; Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura-281 122, Uttar Pradesh, India
Pathak, Juh
 
Uncontrolled Index Term Aging; Goat; Capra hircus
 
Summary, etc. Spermatogonial stem cells (SSCs) have the unique ability of both self-renewing and to produce progeny that undergoes differentiation to spermatozoa. As SSCs exist in very low numbers, therefore efficient in vitro expansion of SSCs is important prior to their clinical applications. In this study, we tried to improve the functionality of putative SSCs (pSSCs) during culture using poly-D-lysine (PDL) coating. For this, plates were coated with 0.01% PDL with different coating time interim treatments (5, 30 and 60 min) while control remained uncoated. The adequate amount of pSSCs of the goat was isolated and enriched using two-step enzymatic digestion and differential plating methods. Further, the functionality of pSSCs was evaluated by cell growth analysis, cell proliferation, senescence, and the presence of pluripotency (alkaline phosphatase, OCT-4) and SSC related (PGP-9.5) markers. The number and size of pSSCs colonies in 0.01% PDL coating groups were significantly (P &lt;0.05) higher than in the control group. Similarly, pSSCs on uncoated plates expressed significantly (P &lt;0.05) higher
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2022-03-03 16:32:24
 
Electronic Location and Access application/pdf
http://op.niscair.res.in/index.php/IJEB/article/view/61049
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 60, ##issue.no## 03 (2022): IJEB [March 2022]
 
Language Note en