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Phylogenetic diversity and predictive functional profile of bacteria associated with marine microalgae, Isochrysis galbana using next generation sequencing

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Title Phylogenetic diversity and predictive functional profile of bacteria associated with marine microalgae, Isochrysis galbana using next generation sequencing
 
Creator Sandhya, SV
Vijayan, KK
 
Subject 16S rDNA
Microalgal-bacterial interaction
Phycosphere bacteria
PICRUSt
Symbiosis
 
Description 223-227
In aquaculture, microalgal-bacterial interaction has ecological
significance, and thus demands better understanding for
improvement of sustainability and productivity of large scale
microalgal cultivation. Here, we assessed the bacterial diversity,
including the uncultivable bacterial assemblage associated with the
marine microalgae Isochrysis galbana using next generation
sequencing approach. Isochrysis has been considered as one of the
most favoured types of live feed in aquaculture and hence, chose
Isochrysis galbana MBTDCMFRI S002. Total genomic DNA was
extracted from I. galbana culture and 16S rDNA V3 region was
sequenced with an Illumina MiSeq platform. A total of 30 different
known bacterial genera were detected from 1190 identified
operational taxonomic units. These bacterial phylotypes were
affiliated to Alphaproteobacteria, Gammaproteobacteria,
Deltaproteobacteria, Actinobacteria, Acidimicrobiia, Bacteroidia,
Flavobacteriia and Bacilli classes. These 30 bacterial genera
comprise only 4.62% of the total OTUs obtained and remaining
95.38% of the sequences do not exhibit any similarity against
known bacterial genera in the taxonomic database. The functional
profile of bacterial communities was predicted using PICRUSt
(Phylogenetic Investigation of Communities by Reconstruction of
Unobserved States) analysis. The results indicated that these
associated bacterial communities are mainly involved in
environmental as well as genetic information processing,
membrane transport and nutrient metabolism. These functions
may mediate their interaction with the phytoplankton host, and
thus improve bacterial survival in microalgal habitat. Overall, the
present study enhances the understanding of microalgal-bacterial
interaction in terms of diversity and functional role of associated
microbial community.
 
Date 2022-03-04T07:07:54Z
2022-03-04T07:07:54Z
2022-03
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/59284
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.60(03) [Mar 2022]