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Non-Saccharomyces yeasts diversity on grape berries and molecular characterization of Starmerella bacillaris yeasts strains having high invertase activity

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Title Non-Saccharomyces yeasts diversity on grape berries and molecular characterization of Starmerella bacillaris yeasts strains having high invertase activity
 
Creator Genç, Tülay Turgut
 
Subject 5.8S rDNA
Vitis vinifera cultivars
Wine fermentation
 
Description 215-222
The diversity of yeast species on grape berries changes depending on various factors. Determination of indigenous yeast
intensity and diversity on grape berries is important for increasing the sensory characteristics of wine as well as fermentation
efficiency. The natural yeast biota of grape berries affects wine flavour and quality by producing some secondary
metabolites and hydrolytic enzymes. Despite the application of different nonconventional yeasts in food and fermentation
industries, many significant researches are conducted in finding and improving the new strains having industrially important
enzyme activities. Invertase enzyme has a vital role in the food industry in which it increases the sweetness of food without
crystallizing them. Here, we studied yeast diversity in grape berries from selected localities and also their invertase activity.
We collected grape berries from Alphonse, Kınalı Yapıncak, Çavuş, Efes Karası, Cinsaut, Atasarısı and Isabella grape
varieties cultivated in Bozcaada island and Gelibolu peninsula. Twenty-one yeast species belonging to seven genera were
identified. The yeast strains having high invertase activities were identified with 5.8S-ITS rDNA sequencing technique. The
diversity of yeast biota on berries collected from Gelibolu was greater than that of Bozcaada. Metschnikowia pulcherrima,
Cryptococcus laurentii and Rhodotorula glutinis yeast species were dominant yeast species on grape berries. A total of 294
sucrose grown yeast strains showed growth on sucrose, and 19 of them exhibit the highest invertase activity that is not
glucose repressible. These 19 yeast strains were identified as Starmerella bacillaris using 5.8S ITS rDNA region and the
phylogenetic analysis was inferred with the Maximum Parsimony method.
 
Date 2022-03-04T07:09:28Z
2022-03-04T07:09:28Z
2022-03
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/59285
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.60(03) [Mar 2022]