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In vitro anticancer activity of ethanolic extract of Stoechospermum marginatum against HT-29 human colon adenocarcinoma cells

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Title In vitro anticancer activity of ethanolic extract of Stoechospermum marginatum against HT-29 human colon adenocarcinoma cells
 
Creator Lakshmanan, Kalaiselvi
Padmanabhan, Sriram
SP, Preetha
RP, Aravindh Babu
M, Parthiban
TA, Kannan
 
Subject Antiproliferative
Apoptosis
Brown algae
Chemotherapeutic
Cytotoxicity
Seaweed extract
 
Description 169-175
Colorectal cancer is a one of the leading causes of death globally and its clinical management of cancer involves
chemotherapy. Increase in the development of resistance to the drugs used in the cancer treatment and serious side effects
associated with chemotherapeutic drugs are the major limitations in cancer therapy. Hence, there exists a huge need to
develop safer natural therapeutic products for cancer therapy. In this study, ethanolic extract of Stoechospermum
marginatum was evaluated for its anticancer activity. The cytotoxicity of S. marginatum extract was evaluated on HT-29
cells by MTT assay. Trypan blue cell viability was also carried out to evaluate cytotoxicity and antiproliferative effect. The
apoptosis-inducing potential of the extract was analyzed by acridine orange and ethidium bromide dual staining method,
mitochondrial membrane potential assay and FITC Annexin V-Propidium iodide staining method. The ethanolic extract of
S. marginatum showed significant dose-dependent cytotoxicity in HT-29 cells Treatment with S. marginatum extract
increased number of apoptotic cells in HT-29 cells and caused damage to mitochondrial membrane potential. The findings
of the present study confirmed in vitro anticancer activity of ethanolic extract S. marginatum
 
Date 2022-03-04T09:45:22Z
2022-03-04T09:45:22Z
2022-03
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/59291
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.60(03) [Mar 2022]