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Effect of poly-D-lysine on proliferation and senescence of caprine spermatogonial stem cells in vitro

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Title Effect of poly-D-lysine on proliferation and senescence of caprine spermatogonial stem cells in vitro
 
Creator Gupta, Deeksha
Kharche, Suresh Dinkar
Singh, Shiva Pratap
Pathak, Juhi
Pathak, Manisha
Chauhan, Manmohan Singh
 
Subject Aging
Goat
Capra hircus
 
Description 161-168
Spermatogonial stem cells (SSCs) have the unique ability of both self-renewing and to produce progeny that undergoes differentiation to spermatozoa. As SSCs exist in very low numbers, therefore efficient in vitro expansion of SSCs is important prior to their clinical applications. In this study, we tried to improve the functionality of putative SSCs (pSSCs) during culture using poly-D-lysine (PDL) coating. For this, plates were coated with 0.01% PDL with different coating time interim treatments (5, 30 and 60 min) while control remained uncoated. The adequate amount of pSSCs of the goat was isolated and enriched using two-step enzymatic digestion and differential plating methods. Further, the functionality of pSSCs was evaluated by cell growth analysis, cell proliferation, senescence, and the presence of pluripotency (alkaline phosphatase, OCT-4) and SSC related (PGP-9.5) markers. The number and size of pSSCs colonies in 0.01% PDL coating groups were significantly (P
 
Date 2022-03-04T09:47:59Z
2022-03-04T09:47:59Z
2022-03
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/59292
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.60(03) [Mar 2022]