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PCR based rapid diagnosis of pathogens

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Relation http://eprints.cmfri.org.in/7879/
 
Title PCR based rapid diagnosis of pathogens
 
Creator Thomas, P C
 
Subject Fish Biotechnology
Fish Diseases
 
Description Use of PCR assisted diagnosis has many advantages over the conventional methods. Since
PCR can amplify oven a single strand of DNA into millions of copies within hours, even a
single bacterial cell collected from the infected fish could be used directly for the
identification. Thus considerable time can be saved.
Two of the PCR assisted diagnostic techniques merits special mention. They are the
DNA fingerprinting based an arbitrarily primed PCR (AP PCR) and amplification of species
specific virulence gene using specific primers flanking these genes. Before going to the
details of these techniques let us examine how the PCR work
 
Date 2003
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/7879/1/B140-24_pc_thomas.pdf
Thomas, P C (2003) PCR based rapid diagnosis of pathogens. In: Winter school on Recent Advances in Mariculture Genetics and Biotechnology, 4- 24 November 2003, Cochin.