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SSR marker-based DNA fingerprinting of Sub1 introgressed lines in the background of traditional rice varieties of Assam India

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Title SSR marker-based DNA fingerprinting of Sub1 introgressed lines in the background of traditional rice varieties of Assam India
 
Creator Gautam, Ashish
Chetia, SK
Sharma, Vinay
Verma, Rahul K
Phukon, Munmi
Kalita, Monalisa
Modi, MK
Ahmed, T
 
Subject DNA Fingerprinting
Rice
SSR
Varietal identification and protection
 
Description 350-356
Rice varieties are usually characterized by agro-morphological descriptors used for seed certification and seed
characterization by following distinctiveness, uniformity, and stability (DUS) test. But in fact, these primary distinguishing
morphological descriptors among rice varieties are very limited and hence face problems to distinguish germplasm
accessions. Germplasm certification in NBPGR requires a DNA fingerprinting profile to explain germplasm uniqueness
compared to existing varieties. Varietal identification has gained a key role worldwide, particularly in plant variety
protection. Sixty-two morphological descriptors studies have shown the Sub1 introgressed advanced lines E-6, C-210,
C-196, 1189-1 and 1160-1 are distinct from the other varieties for more than 15morphological traits, based on these
variations the lines were selected for DNA fingerprinting. About68 SSRs markers were used for DNA fingerprinting in
seven genotypes, two of which were parents (Ranjit, Bahadur) and three Sub1 introgressed advanced lines (E6, C210, C196)
in Ranjit background, and two Sub1 introgressed advanced lines (1189-1, 1160-1) in Bahadur background. DNA
fingerprinting was done on these genotypes of rice using SSR markers. Among the 68 SSR markers, total 65 markers were
amplified and three were found not amplified. Out of 65 markersfour of them viz. RM 152, RM 172, RM 251, and RM 346
showed better polymorphism with amplicon size ranges from 155-163 bp, 150-159 bp, 137-147 bp, and 166-175 bp,
respectively, and remaining 61 showed monomorphic amplification. Therefore, SSR (Simple-sequence repeats) based DNA
fingerprinting helped to differentiate Ranjit, Bahadur, E-6, C-210, C-196, 1189-1, and 1160-1. Hence, the research reveals
that newly developed high-yielding Sub1 introgressed advanced lines in the background of traditional Assam rice varieties
(Ranjit and Bahadur) are unique in their identity.
 
Date 2022-03-25T11:29:49Z
2022-03-25T11:29:49Z
2022-03
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscair.res.in/handle/123456789/59381
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.59(3) [March 2022]