Blood formed elements of the women with uterine tumors as one of the criterion for assessment of severity of the pathology
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Title |
Blood formed elements of the women with uterine tumors as one of the criterion for assessment of severity of the pathology
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Creator |
Kotrikadze, Nanuli
Tuphinashvili, Tamar Alibegashvili, Manana Ramishvili, Liana Nakashidze, Irina Artmeladze, Mukhamed Gordeziani, Manana Zibzibadze, Maia Khazaradze, Ana Sepiashvili, Bela Ahmad, Sarfraz |
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Subject |
Blood Formed Elements
Hormonal Imbalance Menopausal Women Morphological Characteristics Pathological Assessments Transmission Electron Microscopy Uterine Cancer |
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Description |
268-275
The indicators for structural analysis of blood formed elements are prominent in the assessment of pathologies, diagnostics and the degree. Therefore, we aimed to evaluate the ongoing alterations that reflect on the structural characteristics of blood formed elements based on the hormonal imbalance among menopausal women with uterine tumors. Blood samples from the women with benign (n=20), malignant (n=20) uterine tumors, and healthy menopausal women (control, n=20) were used. Enzyme-linked Immunosorbent assay (ELISA) kits were used for the quantitative determination of hormones. The blood formed elements ultrastructure observations were conducted using transmission electron microscope. Compared to control (33.8±0.7 pg/mL), estradiol level was higher in benign (45.7±0.9 pg/mL) and malignant (70.7±3.7 pg/mL) cases (P< 0.001). Similar pattern was noted in testosterone levels [control=0.38±0.03 ng/mL, benign=0.55±0.04 ng/mL (P< 0.01), malignant=1.56±0.14 ng/mL (P< 0.001)] was higher in malignant cases. In contrast, progesterone levels were decreased in the disease cases [control=0.93±0.05 ng/mL, benign=0.44±0.003 ng/mL, malignant=0.31±0.02 ng/ml (P< 0.001)]. Assessments of the morphologic structure of erythrocytes revealed pathological forms of erythrocytes (poikilocytosis) in case of benign, as well as in malignant tumors. particularly target cells (codocytes), hamlet cells, teardrop cells (dacrocytes), sickle cell (drepanocytes) erythrocytes. Using ELISA and transmission electron microscopy our results demonstrate that in case of malignant uterine tumor quantitative/structural changes occur in blood formed elements indicating ongoing alterations in hormonal imbalance. Assessing these changes in structural characteristics would be useful in examining uterine pathologies and subsequent treatment plans. |
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Date |
2022-03-25T11:51:05Z
2022-03-25T11:51:05Z 2022-03 |
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Type |
Article
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Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscair.res.in/handle/123456789/59388 |
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Language |
en
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Publisher |
NIScPR-CSIR, India
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Source |
IJBB Vol.59(3) [March 2022]
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