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Discerning the role of a functional arsenic- resistance cassette in the evolution and adaptation of a rice pathogen

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Discerning the role of a functional arsenic- resistance cassette in the evolution and adaptation of a rice pathogen
 
Creator Kaur, Amandeep
Rana, Rekha
Saroha, Tanu
Patil, Prabhu B.
 
Subject QR Microbiology
 
Description Arsenic is highly toxic element to all forms of life and is a major environmental contaminant. Understanding acquisition, detoxification and adaptation mechanisms in bacteria that are associated with the host in arsenic-rich conditions can provide novel insights into the evolutionary dynamics of host–microbe–environment interactions. In the present study, we have investigated an arsenic-resistance mechanism acquired during the evolution of a particular lineage in the population of Xanthomonas oryzae pv. oryzae, which is a serious plant pathogen infecting rice. Our study revealed the horizontal acquisition of a novel chromosomal 12 kb ars cassette in X. oryzae pv. oryzae IXO1088 that confers high resistance to arsenate/arsenite. The ars cassette comprises several genes that constitute an operon induced in the presence of arsenate/arsenite. Transfer of the cloned ars cassette to X. oryzae pv. oryzae BXO512, which lacks the cassette, confers an arsenic-resistance phenotype. Furthermore, the transcriptional response of X. oryzae pv. oryzae IXO1088 under arsenate/arsenite exposure was analysed using RNA sequencing. Arsenic detoxification and efflux, oxidative stress, iron acquisition/storage, and damage repair are the main cellular responses to arsenic exposure. Our investigation has provided insights into the existence of a novel detoxification and adaptation mechanism within the X. oryzae pv. oryzae population to deal with high-arsenic conditions outside the rice plant.
 
Publisher Microbiology Society
 
Date 2021-07-13
 
Type Article
PeerReviewed
 
Identifier Kaur, Amandeep and Rana, Rekha and Saroha, Tanu and Patil, Prabhu B. (2021) Discerning the role of a functional arsenic- resistance cassette in the evolution and adaptation of a rice pathogen. MICROBIAL GENOMICS, 7 (7).
 
Relation http://crdd.osdd.net/open/2712/