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Polyphasic characterization of and genomic insights into a haloalkali-tolerant Saccharibacillus alkalitolerans sp. nov., that produces three cellulase isozymes and several antimicrobial compounds

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Polyphasic characterization of and genomic insights into a haloalkali-tolerant Saccharibacillus alkalitolerans sp. nov., that produces three cellulase isozymes and several antimicrobial compounds
 
Creator Darji, Himanshu
Verma, Neha
Lugani, Yogita
Mehrotra, Priyam
Sindhu, Dev Kant
Vemuluri, Venkata Ramana
 
Subject QR Microbiology
 
Description A cellulase producing novel bacterial strain VR-M41T was isolated from an open-air vegetable and fruit market. Cells are found to be rod-shaped, endospore forming, positive for Gram’s stain and negative for catalase, oxidase and urease. Strain VR-M41T was halotolerant (upto 8.0% NaCl, w/v), motile and facultative anaerobe. It grew at wide range of pH (6.0–10.0) and temperatures (20–40 °C). Strain VR-M41T produced three isozymes of Carboxymethylcellulase. The 16S rRNA gene sequence of strain VR-M41T was 97.3% similar to both Saccharibacillus kuerlensis DSM 22868T and Saccharibacillus sacchari DSM 19268T, and less than 96.4% with the rest of the valid species of the genus Saccharibacillus. Whole-genome ANI, dDDH and genome phylogenetic tree analysis revealed that strain VR-M41T significantly differed from Saccharibacillus kuerlensis DSM 22868T and Saccharibacillus sacchari DSM 19268T (ANI 79.6–79.7% and dDDH 23.1%). The strain comprised of MK-7 and anteiso-C 15:0 (42.2%) as predominant isoprenoid quinone and fatty acid respectively. Major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The draft genome of strain VR-M41T consisted of 5,386,426 base pairs with 5103 annotated genes, out of which 2147 corresponded to hypothetical proteins and 2956 with functional assignments. Pan-genome analysis revealed the presence of 2998 core genes, 828 accessory genes, and 1131 unique genes of Saccharibacillus. Strain VR-M41T produced antimicrobials against Staphylococcus aureus, Streptococcus pneumoniae, Micrococcus luteus and Shigella flexneri. Significant phenotypic and genotypic differentiating characteristics from closely related species, indicated that strain VR-M41T is a novel species of the genus Saccharibacillus, for which the name Saccharibacillus alkalitolerans sp. nov., is proposed. The type strain is VR-M41T (= KCTC 43183T=NBRC 114337T).
 
Publisher Springer
 
Date 2021-04-28
 
Type Article
PeerReviewed
 
Relation https://link.springer.com/article/10.1007/s10482-021-01575-x
http://crdd.osdd.net/open/2727/
 
Identifier Darji, Himanshu and Verma, Neha and Lugani, Yogita and Mehrotra, Priyam and Sindhu, Dev Kant and Vemuluri, Venkata Ramana (2021) Polyphasic characterization of and genomic insights into a haloalkali-tolerant Saccharibacillus alkalitolerans sp. nov., that produces three cellulase isozymes and several antimicrobial compounds. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 114 (7). pp. 1043-1057.