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Cumulative Signaling Through NOD-2 and TLR-4 Eliminates the Mycobacterium Tuberculosis Concealed Inside the Mesenchymal Stem Cells

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Cumulative Signaling Through NOD-2 and TLR-4 Eliminates the Mycobacterium Tuberculosis Concealed Inside the Mesenchymal Stem Cells
 
Creator Aqdas, Mohammad
Singh, Sanpreet
Amir, Mohammed
Maurya, Sudeep Kumar
Pahari, Susanta
Agrewala, Javed Naim
 
Subject QR Microbiology
 
Description For a long time, tuberculosis (TB) has been inflicting mankind with the highest morbidity and mortality. Although the current treatment is extremely potent, a few bacilli can still hide inside the host mesenchymal stem cells (MSC). The functional capabilities of MSCs are known to be modulated by TLRs, NOD-2, and RIG-1 signaling. Therefore, we hypothesize that modulating the MSC activity through TLR-4 and NOD-2 can be an attractive immunotherapeutic strategy to eliminate the Mtb hiding inside these cells. In our current study, we observed that MSC stimulated through TLR-4 and NOD-2 (N2.T4) i) activated MSC and augmented the secretion of pro-inflammatory cytokines; ii) co-localized Mtb in the lysosomes; iii) induced autophagy; iv) enhanced NF-κB activity via p38 MAPK signaling pathway; and v) significantly reduced the intracellular survival of Mtb in the MSC. Overall, the results suggest that the triggering through N2.T4 can be a future method of immunotherapy to eliminate the Mtb concealed inside the MSC.
 
Publisher Frontiers
 
Date 2021-07-07
 
Type Article
PeerReviewed
 
Relation https://www.frontiersin.org/articles/10.3389/fcimb.2021.669168/full
http://crdd.osdd.net/open/2758/
 
Identifier Aqdas, Mohammad and Singh, Sanpreet and Amir, Mohammed and Maurya, Sudeep Kumar and Pahari, Susanta and Agrewala, Javed Naim (2021) Cumulative Signaling Through NOD-2 and TLR-4 Eliminates the Mycobacterium Tuberculosis Concealed Inside the Mesenchymal Stem Cells. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 11.