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N-hexanoyl-L-homoserine lactone-degrading Pseudomonas aeruginosa PsDAHP1 protects zebrafish against Vibrio parahaemolyticus infection

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Relation http://eprints.cmfri.org.in/10386/
 
Title N-hexanoyl-L-homoserine lactone-degrading Pseudomonas aeruginosa
PsDAHP1 protects zebrafish against Vibrio parahaemolyticus infection
 
Creator Gopalakrishnan, Vinoj
Jayakumar, R
Chen, Jiann-Chu
Withyachumnarnkul, Boonsirm
Shanthi, Sathappan
Vaseeharan, B
 
Subject Fish Biotechnology
Fish Diseases
 
Description Four strains of N-hexanoyl-L-homoserine lactone (AHL)-degrading Pseudomonas spp., named PsDAHP1,
PsDAHP2, PsDAHP3, and PsDAHP4 were isolated and identified from the intestine of Fenneropenaeus
indicus. PsDAHP1 showed the highest AHL-degrading activity among the four isolates. PsDAHP1 inhibited
biofilm-forming exopolysaccharide and altered cell surface hydrophobicity of virulent green fluorescent
protein (GFP)-tagged Vibrio parahaemolyticus DAHV2 (GFP-VpDAHV2). Oral administration of PsDAHP1
significantly reduced zebrafish mortality caused by GFP-VpDAHV2 challenge, and inhibited colonisation
of GFP-VpDAHV2 in the gills and intestine of zebrafish as evidence by confocal laser scanning microscope
and selective plating. Furthermore, zebrafish receiving PsDAHP1-containing feed had increased phagocytic
cells of its leucocytes, increased serum activities of superoxide dismutase and lysozyme. The results
suggest that Pseudomonas aeruginosa PsDAHP1 could protect zebrafish from V. parahaemolyticus infection
by inhibiting biofilm formation and enhancing defence mechanisms of the fish.
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/10386/1/Fish%26_SF_immunology_2015.pdf
Gopalakrishnan, Vinoj and Jayakumar, R and Chen, Jiann-Chu and Withyachumnarnkul, Boonsirm and Shanthi, Sathappan and Vaseeharan, B (2015) N-hexanoyl-L-homoserine lactone-degrading Pseudomonas aeruginosa PsDAHP1 protects zebrafish against Vibrio parahaemolyticus infection. Fish & Shellfish Immunology, 42. pp. 204-212.