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Synergistic effect of folic acid and galantamine against experimentally induced oxidative stress in IMR 32 cells

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Title Synergistic effect of folic acid and galantamine against experimentally induced oxidative stress in IMR 32 cells
 
Creator Upadhyay, Shagun Dubey
Ahmad, Yusra
Kohli, Seema
Sharma, RK
 
Subject Galanthus nivalis
Nootropic
Neuroprotection
Snowdrop
 
Description 286-292
Galantamine is an active constituent obtained from Galanthus
nivalis L., a traditional herb known for its pharmacological
properties, particularly nootropic effect. Folic acid is a dietary
supplement that enhances neuronal activity. Effect of galantamine
and folic acid on human neuronal cells is well known. In the present
study, we explored the protective effect of galantamine and folic
acid, both independently as well as in combination, over antioxidant
defence system and nootropic effects on human neuroblastoma cells
IMR-32. The treatment galantamine, folic acid and their
combination was given for 24 h and cytotoxicity study was carried
out by trypan blue dye exclusion assay. Apoptosis and necrosis
were observed using Propidium iodide (PI) and Hoechst double
staining method. Biochemical assays viz. total protein, protein
carbonyl, lipid peroxidation and glutathione were analyzed along
with super oxide dismutase and catalase. Result of cytotoxicity
showed dose dependent increase in percent viability and significant
decrease was observed in apoptosis and necrosis. Moreover,
exposure to Galantamine, Folic acid and their combination
significantly decreased lipid peroxidation and protein carbonyl
formation along with the enhancement in antioxidant defence
mechanism. Findings of these dose reliant toxicity study of
Galantamine , Folic acid and their combination suggest that these
has higher potency when given together and shows synergistic
effect. They also causes repair of human neuronal cells IMR-32
cells enhancing the cell viability and consumption of Galantamine
and Folic acid together will help in prevention of CNS disorders and
neurodegeneration.
 
Date 2022-03-31T08:59:53Z
2022-03-31T08:59:53Z
2022-04
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/59410
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.60(04) [April 2022]