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Extremely low frequency magnetic exposure attenuates oxidative stress and apoptotic cell death in injured spinal cord of rats

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Title Extremely low frequency magnetic exposure attenuates oxidative stress and apoptotic cell death in injured spinal cord of rats
 
Creator Ambalayam, Sajeev
Mathur, Rashmi
 
Subject Apoptosis
Spinal cord injury (SCI)
TUNEL
 
Description 248-257
Secondary spinal cord injury (SCI) sets on immediately after trauma which results in vascular, morphological and
biochemical changes at the site of lesion. Amongst these, the crucial events such as oxidative stress and apoptosis result in
spreading the injury to adjacent tissues following the initial insult. Exposure to extremely low frequency magnetic field
(ELFMF) is reported to modulate oxidative stress and cell death in vivo. Here, we investigated the influence of ELFMF on
oxidative stress and cell death after SCI. Adult male Wistar rats were divided into Sham-SCI (Laminectomy only),
Sham-SCI+MF (ELFMF exposure; 17.96 μT intensity, 50 Hz frequency, 2 h duration/day to Sham-SCI rats), SCI (complete
transection of T13 spinal cord) and SCI+MF (ELFMF exposure to SCI rats) groups. They were sacrificed on day 2 for
antioxidant assay and day 1, 2 and 3 for cell death study. The oxidative stress was assessed by antioxidant enzyme activities
and the cell death was assessed by TUNEL assay and Caspase-3 immnuohistochemistry. Data revealed significant reduction
in the lipid peroxidation and number of cells undergone cell death besides improvement in antioxidant levels in the spinal
cord of ELFMF exposed SCI rats. These novel findings demonstrate the potential of ELFMF to attenuate oxidative stress
and cell death in the early stage of secondary injury process after SCI.
 
Date 2022-03-31T09:57:11Z
2022-03-31T09:57:11Z
2022-04
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscair.res.in/handle/123456789/59415
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.60(04) [April 2022]