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Production of Aspergillus quadrilineatus MT083999 Chitinase, β-1,3-Glucanase and Nano-silver Important for Biocontrol of Fusarium spp. Infecting Crops

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Title Production of Aspergillus quadrilineatus MT083999 Chitinase, β-1,3-Glucanase and Nano-silver Important for Biocontrol of Fusarium spp. Infecting Crops
 
Creator Aty, Abeer A Abd El
E Ashour, Warda
 
Subject Fungi
Inhibition zone diameter
Phytopathogens
Statistical designs
 
Description 416-425
In our search for an active biocontrol agent against Fusarium solani and F. oxysporum, the major species causing wilt, root
rot and damping-off in plants affecting crops. The fungal isolate genetically identified as Aspergillus quadrilineatus MT083999
displayed high antagonistic activities against the tested root-rot fungi, and presented valuable chitinase and β-glucanase
activities of 235.484 and 508.953 U/gds, respectively. Solid state fermentation conditions were optimized by applying 20 trails
of the central composite design (Three-factor-five-level) and two responses of chitinase R1 and β-glucanase R2 activities,
respectively. The optimum activities of chitinase (386.70 U/gds) and β-glucanase (1094.70 U/gds) were obtained in trial 4 and 2
respectively, with about 1.64-fold increase in chitinase activity and 2.15-fold increase in β-glucanase activity when compared
with initial fermentation. Biosynthesis of nano-silver from A. quadrilineatus was evaluated in 10 experiments of another design
of 2-factors-5-levels. The antifungal effects of each biosynthesized nano-silver against F. solani (R1) and F. oxysporum (R2)
were evaluated as Inhibition Zone Diameter (IZD).The high fungicide nano-silver against both tested phytopathogens was
characterized by UV-visible spectrophotometry, Transmission Electron Microscopy (TEM), Zeta Dynamic Light Scatter (DLS)
and Fourier Transform Infrared Spectroscopy (FTIR). The results indicated that A. quadrilineatus MT083999 chitinolytic
enzymes and nano-silver can be applied as a novel candidate in biocontrol of Fusarium wilt infecting crops worldwide.
 
Date 2022-04-05T11:32:20Z
2022-04-05T11:32:20Z
2022-04
 
Type Article
 
Identifier 0975-1084 (Online); 0022-4456 (Print)
http://nopr.niscair.res.in/handle/123456789/59431
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source JSIR Vol.81(04) [April 2022]