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<p>Production of <em>Aspergillus quadrilineatus</em> MT083999 Chitinase, β-1,3-Glucanase and Nano-silver Important for Biocontrol of <em>Fusarium</em> spp<em>.</em> Infecting Crops</p>

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Title Statement <p>Production of <em>Aspergillus quadrilineatus</em> MT083999 Chitinase, β-1,3-Glucanase and Nano-silver Important for Biocontrol of <em>Fusarium</em> spp<em>.</em> Infecting Crops</p>
 
Added Entry - Uncontrolled Name Abd El Aty, Abeer ; Department of Biology, College of Science, University of Hafr Al Batin, P.O. Box 1803, Hafr Al Batin, Saudi Arabia
Ashour, Warda E; Chemistry of Natural and Microbial Products department, National Research Centre, Dokki, Giza, 12622, Egypt
National Research Centre
 
Uncontrolled Index Term Fungi, Inhibition zone diameter, Phytopathogens, Statistical designs
 
Summary, etc. <p>In our search for an active biocontrol agent against <em>F. solani</em> and <em>F. oxysporum</em>, the major species causing wilt, root rot and damping-off in plants affecting crops. The fungal isolate genetically identified as <em>Aspergillus quadrilineatus</em> MT083999<strong> </strong>displayed high antagonistic activities against the tested root-rot fungi, and presented valuable chitinase and β-glucanase activities of 235.484 and 508.953 U/gds, respectively. Solid state fermentation conditions were optimized by applying Three–factor–five–level central composite design (CCD) with twenty experiments and two responses of chitinase R1 and β-glucanase R2 activities, respectively. The optimum activities of chitinase (386.70 U/gds) and β-glucanase (1094.70 U/gds) were obtained in trial 4 and 2 respectively, with about 1.64-fold increase in chitinase activity and 2.15-fold increase in β-glucanase activity when compared with initial fermentation. Biosynthesis of nano-silver from <em>A. quadrilineatus</em><strong> </strong>was evaluated in 10 experiments of another design of 2–factors–5–levels. The antifungal effects of each biosynthesized nano-silver against <em>F.</em> <em>solani</em> (R1) and <em>F. oxysporum</em> (R2)<strong> </strong>were evaluated as Inhibition zone diameter (IZD). The high fungicide nano-silver against both tested phytopathogens was characterized by UV–visible spectrophotometry, transmission electron microscopy (TEM), Zeta dynamic light scatter (DLS) and Fourier transform infrared spectroscopy (FTIR). The results indicated that<em> A. quadrilineatus</em> MT083999 chitinolytic enzymes and nano-silver can be applied as a novel candidate in biocontrol of Fusarium wilt infecting crops worldwide.</p>
 
Publication, Distribution, Etc. Journal of Scientific and Industrial Research (JSIR)
2022-04-08 17:32:38
 
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http://op.niscair.res.in/index.php/JSIR/article/view/46479
 
Data Source Entry Journal of Scientific and Industrial Research (JSIR); ##issue.vol## 81, ##issue.no## 04 (2022): Journal of Scientific and Industrial Research
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/JSIR/article/download/46479/465549428
http://op.niscair.res.in/index.php/JSIR/article/download/46479/465549429
http://op.niscair.res.in/index.php/JSIR/article/download/46479/465549431
http://op.niscair.res.in/index.php/JSIR/article/download/46479/465549432