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Development of biofilm of Vibrio alginolyticus for oral immunostimulation of shrimp

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Relation http://eprints.cmfri.org.in/9885/
 
Title Development of biofilm of Vibrio alginolyticus for oral
immunostimulation of shrimp
 
Creator Sharma, S R Krupesha
Shankar, K M
Sathyanarayana, M L
Patil, Rajreddy
Narayanaswamy, H D
Rao, Suguna
 
Subject Fish Biotechnology
 
Description Biofilm (BF) of Vibrio alginolyticus was developed on chitin flakes. BF formation
was studied at various nutrient concentrations and incubation time. Highest colonyforming
units of BF cells were obtained with 0.15% trypticase soya broth and at 3 days of
incubation. The BF cells could be completely inactivated at 80C in 10 min and with 10%
formalin in 24 h. SDS–PAGE profile of BF cells revealed repression of four proteins and
expression of three new proteins compared to free cells (FC). The preliminary immune
response studies showed that BF cells were superior to FC in stimulating the non-specific
immune response of Penaeus monodon
 
Publisher Kluwer
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/9885/1/Aquaculture_International_Sharma.pdf
Sharma, S R Krupesha and Shankar, K M and Sathyanarayana, M L and Patil, Rajreddy and Narayanaswamy, H D and Rao, Suguna (2011) Development of biofilm of Vibrio alginolyticus for oral immunostimulation of shrimp. Aquaculture International, 19 (3). pp. 421-430.