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View Archive Info| Field | Value | |
| Title |
Dr
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| Creator |
keshavarz, fatemeh
Ghalamfarsa, Ghasem Dorfaki, Maryam Bardania, Hasan Khosravani, Fatemeh Nazari, Zeynab |
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| Subject |
Cancer, Quercetin, CRC, EGFR, Nanoliposomes, Cytotoxicity
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| Description |
16
Background: EGFR is one of the tyrosine kinase receptors increasingly expressed in colorectal cancer (CRC) cells and its inhibition is effective in cancer treatment. Quercetin is also a flavonoid with anti-cancer effects. However, its pharmaceutical applications are limited by low stability, insufficient bioavailability, poor solubility, and poor permeability. Objectives: This study was aimed to enhance the cytotoxicity of quercetin against SW48 colorectal cancer cells and evaluate its effect on inhibition of EGFR gene expression. Materials and Methods: the lipid thin-film hydration method was used to synthesize quercetin-loaded liposomes, and characterization was done via TEM and DLS, and HPLC analyses. Then, cytotoxicity of quercetin-loaded liposomes on the CRC SW48 cell line, as well as the apoptosis incidence, and the expression of the EGFR gene in these cells, was investigated by MTT, flow cytometry, and real-time PCR. Results: The produced nanoparticles were spherical, uniform, and in size of 150±10 nm. HPLC analysis revealed a 98% loading capacity for quercetin. Although both free quercetin and loaded on liposomes showed considerable cytotoxicity against cancer cells, the activity of combined form was much higher. So that 50 µg.L-1 of this compound reduced the viability of SW48 cells by more than 80% (IC50: 10.6 µg.L-1), while the viability of free quercetin-treated cells was 66% (IC50: 18.7 µg.L-1). The apoptosis induced in the cells was roughly equal (38.91% versus 37.58%). However, the EGFR gene expression was significantly lower in cells treated with quercetin-loaded liposomes compared to the quercetin alone. Conclusions: Quercetin in combination with nanoliposomes presents a superior ability to inhibit cell proliferation, induce apoptosis, and inhibit EGFR expression than free quercetin. Yasuj University of Medical Sciences, Yasuj, Iran |
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| Date |
2022-04-17T18:31:58Z
2022-04-17T18:31:58Z 2022-03-17 |
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| Type |
Article
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| Identifier |
2582-6492 (Online); 0036-8512 (Print)
http://nopr.niscair.res.in/handle/123456789/59530 |
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| Language |
en
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| Publisher |
NIScPR-CSIR, India
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| Source |
SR Vol.59(04) [April 2022]
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