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Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice
 
Creator Dubey, Ashish Kumar
Podia , Mansi
Priyanka, Priyanka
Raut , Sachin
Singh, Sanpreet
Pinnaka , Anil Kumar
Khatri , Neeraj
 
Subject QR Microbiology
 
Description Lactiplantibacillus plantarum MTCC 2621 is a well-characterized probiotic strain and is reported to possess many health benefits. However, the wound healing potential of this probiotic is yet to be explored. Here, we have assessed the antibacterial, antioxidant, and wound healing activities of cell-free supernatant of Lactiplantibacillus plantarum MTCC 2621 (Lp2621). Lp2621 exhibited excellent antibacterial activity against the indicator bacteria in the agar well diffusion assay. Lp2621 did not show any hemolytic activity. The safety of Lp2621 gel was established using the skin irritation assay in BALB/c mice, and no dermal reactions were observed. The supernatant showed 60-100% protection of A549 cells against H2O2-induced stress. In the scratch assay, Lp2621 accelerated wound healing after 24 h of treatment. The percent wound healing was significantly higher in cells treated with Lp2621 at 18-24 h posttreatment. In an excision wound healing in mice, topical application of Lp2621 gel showed faster healing than the vehicle- and betadine-treated groups. Similar wound healing activity was observed in wounds infected with Staphylococcus aureus. Histological examination revealed better wound healing in Lp2621-treated mice. Topical treatment of the wounds with Lp2621 gel resulted in the upregulation of pro-inflammatory cytokine IL-6 in the early phase of wound healing and enhanced IL-10 expression in the later phase. These findings unveil a protective role of Lp2621 against bacterial infection, oxidative stress, and wound healing.
 
Publisher Frontiers Media S.A
 
Date 2021-11-04
 
Type Article
PeerReviewed
 
Relation https://www.frontiersin.org/articles/10.3389/fphar.2021.728614/full
http://crdd.osdd.net/open/2949/
 
Identifier Dubey, Ashish Kumar and Podia , Mansi and Priyanka, Priyanka and Raut , Sachin and Singh, Sanpreet and Pinnaka , Anil Kumar and Khatri , Neeraj (2021) Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice. Frontiers in pharmacology. ISSN 1663-9812