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Characterization and Antimicrobial Studies of Iturin-Like and Bogorol-Like Lipopeptides From Brevibacillus spp. Strains GI9 and SKDU10

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Characterization and Antimicrobial Studies of Iturin-Like and Bogorol-Like Lipopeptides From Brevibacillus spp. Strains GI9 and SKDU10
 
Creator Singh, S.S
Sharma , Deepika
Baindara, Piyush
Choksket, Stanzin
Harshvardhan, Harshvardhan
Mandal, Santi M
Grover, Vishakha
Korpole, Suresh
 
Subject QR Microbiology
 
Description Accession numbers for whole-genome sequence of Brevibacillus sp. strain GI9 and SKDU10 are CAGD01000001 to CAGD01000061 and LSSO00000000, respectively. Members of the genus Brevibacillus have been demonstrated to produce a variety of bioactive compounds including polyketides, lipopeptides and bacteriocins. Lipopeptides are non-ribosomally synthesized surface-active compounds with antimicrobial, antitumor, and immune-stimulatory activities. They usually exhibit strong antifungal and antibacterial activities and are considered as promising compounds in controlling fungal diseases. In this study, we have characterized two lipopeptides from Brevibacillus sp. strains GI9 and SKDU10. The corresponding lipopeptides were purified by reverse-phase high-performance liquid chromatography. Mass analysis and characterization by MALDI-TOF-MS (Matrix-assisted laser desorption ionization time-of-flight mass spectrometry) analysis revealed production of an iturin-like lipopeptide by strain GI9 and bogorol-like lipopeptide by strain SKDU10. Both lipopeptides exhibited broad spectrum antibacterial activity and inhibited the growth of various fungi. They showed minimum inhibitory concentration (MIC) values between 90 and 300 μg/ml against indicator strains of bacteria and drug-resistant Candida indicator strains. The lipopeptides did not show phytotoxic effect in seed germination experiments but caused hemolysis. Further, both lipopeptides inhibited the growth of fungi on fruits and vegetables in in vitro experiments, thereby exhibited potential use in biotechnological industry as effective biocontrol agents.
 
Publisher Frontiers Media S.A
 
Date 2021-10-29
 
Type Article
PeerReviewed
 
Relation https://www.frontiersin.org/articles/10.3389/fmicb.2021.729026/full
http://crdd.osdd.net/open/2960/
 
Identifier Singh, S.S and Sharma , Deepika and Baindara, Piyush and Choksket, Stanzin and Harshvardhan, Harshvardhan and Mandal, Santi M and Grover, Vishakha and Korpole, Suresh (2021) Characterization and Antimicrobial Studies of Iturin-Like and Bogorol-Like Lipopeptides From Brevibacillus spp. Strains GI9 and SKDU10. Frontiers in microbiology. ISSN 1664-302X