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Rapid identification of Vibrio harveyi isolates in Panulirus homarus

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Relation http://eprints.cmfri.org.in/10329/
http://www.ijcmas.com/Archives/vol-2-3/V.A.Leslie%20etal.pdf
 
Title Rapid identification of Vibrio harveyi isolates in Panulirus homarus
 
Creator Leslie, V A
Margaret Muthu Rathinam, A
Balasingh, Anusha
 
Subject Microbiology
Crustacean Fisheries
 
Description Vibrio is the most common genera associated with Crustaceans often causing
significant economic losses. Many Vibrio species are pathogenic to human and have
been implicated in the food borne disease. Tail fan necrosis is of recognized constraint
in lobsters live holding industry because of reduction in value of affected lobsters.
Aquaculture has an important role in the development of many national economics and
play a key role in rural development. As the expansion of aquaculture product, there is
a concern over the impact of aquaculture on the environmental sustainability and also
over the requirements on quality and food safety for consumers and regulators. For this
reason, it is a need to improve aquaculture technology and management system to
address the need for the eco-friendly production process and food safety concerns in
sustainability of national aquaculture. In the present study a total of 11 luminescent
bacteria were isolated from the infected Indian spiny lobster Panulirus homarus. Many
of the lobsters in the holding facilities developed a range of exoskeleton and tail
lesions. The splitting and crackling of the chitin could have allowed the entrance of the
bacteria such as Vibrio harveyi, commonly present in water. Predisposing factor could
have included handling of the animals, the stress of holding resulting in decreased
immunocompetence, injury from fighting or abrasions from the cage wire and elevated
water temperatures during the period of holding, V. harveyi is an important pathogen
and is extremely difficult to identify because it is phenotypically diverse. Hence PCR
technique was employed using 16S rDNA sequences to reduce the duration of
identification of this species, a valuable tool for a rapid and accurate detection and
hence earlier treatment can be administered which may increase the survival rate from
vibriosis
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/10329/1/IJCMAS_Leslie.pdf
Leslie, V A and Margaret Muthu Rathinam, A and Balasingh, Anusha (2013) Rapid identification of Vibrio harveyi isolates in Panulirus homarus. International Journal of Current microbiology and Applied Sciences, 2 (3). pp. 6-10.