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Development of marine fish cell lines and stem cell lines: applications in mariculture and marine biodiversity

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Relation http://eprints.cmfri.org.in/10432/
 
Title Development of marine fish cell lines
and stem cell lines: applications in
mariculture and marine biodiversity
 
Creator Sobhana, K S
 
Subject Mariculture
Microbiology
Marine Biodiversity
 
Description In vitro transformed/continuous marine fish cell lines are
important for virology, gene expression studies, cytogenetics,
as in vitro models in toxicology, transgenics, in many other
basic studies and in biodiversity conservation. In vitro
cell culture systems/cell lines derived from marine fish are
necessary for isolation and characterisation of viruses and
studies on diversity of viruses in marine environment.
The main impetus for the development of many of the
continuous fish cell lines was to provide the means for
isolating and identifying viruses that are the causative
agents of epizootics of commercially important species.
Unlike other microorganisms, which can be readily grown in
artificial nutrient medium, viruses are obligatory intracellular
pathogens and their isolation and propagation are totally
dependent on the availability of a live host, such as permissive
cell cultures. In addition, most viruses are host-specific and
tissue-specific, and they can only be isolated and propagated
using cell lines established from tissues of the same/related
host species. An appropriate cell line is the most important
laboratory tool to grow, isolate, characterise, and identify
pathogenic fish viruses. With few exceptions, fish viruses can
be replicated only in fish cell cultures.
 
Publisher CMFRI; Kochi
 
Date 2015
 
Type Teaching Resource
NonPeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/10432/1/28_Sobhana_KS.pdf
Sobhana, K S (2015) Development of marine fish cell lines and stem cell lines: applications in mariculture and marine biodiversity. [Teaching Resource]