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Recent advancements in CRISPR/Cas technology for accelerated crop improvement

OAR@ICRISAT

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Relation http://oar.icrisat.org/11983/
https://doi.org/10.1007/s00425-022-03894-3
doi:10.1007/s00425-022-03894-3
 
Title Recent advancements in CRISPR/Cas technology for accelerated crop improvement
 
Creator Das, D
Singha, D L
Paswan, R R
Chowdhury, N
Sharma, M
Reddy, P S
Chikkaputtaiah, C
 
Subject Crop Improvement
Genetic Engineering
Genetics and Genomics
 
Description The likelihood of reduced agricultural production due to highly turbulent climatic conditions increases as the
global population expands. The second paradigm of stress-resilient crops with enhanced tolerance and increased productivity against various stresses is paramount to support global production and consumption equilibrium. Although traditional breeding approaches have substantially increased crop production and yield, effective strategies are anticipated to restore crop productivity even further in meeting the world’s increasing food demands. CRISPR/Cas, which originated in prokaryotes, has surfaced as a coveted genome editing tool in recent decades, reshaping plant molecular biology in unprecedented ways
and paving the way for engineering stress-tolerant crops. CRISPR/Cas is distinguished by its efficiency, high target specificity, and modularity, enables precise genetic modification of crop plants, allowing for the creation of allelic variations in the germplasm and the development of novel and more productive agricultural practices. Additionally, a slew of advanced biotechnologies
premised on the CRISPR/Cas methodologies have augmented fundamental research and plant synthetic biology
toolkits. Here, we describe gene editing tools, including CRISPR/Cas and its imitative tools, such as base and prime editing, multiplex genome editing, chromosome engineering followed by their implications in crop genetic improvement. Further, we comprehensively discuss the latest developments of CRISPR/Cas technology including CRISPR-mediated gene drive, tissue-specific genome editing, dCas9 mediated epigenetic modification and programmed self-elimination of transgenes in plants. Finally, we highlight the applicability and scope of advanced CRISPR-based techniques in crop genetic improvement.
 
Publisher Springer
 
Date 2022-03
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11983/1/Das2022_Article_RecentAdvancementsInCRISPRCasT.pdf
Das, D and Singha, D L and Paswan, R R and Chowdhury, N and Sharma, M and Reddy, P S and Chikkaputtaiah, C (2022) Recent advancements in CRISPR/Cas technology for accelerated crop improvement. Planta (TSI), 255 (5). pp. 1-27. ISSN 0032-0935