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Cloning, expression and characterization of L-arabinose isomerisefrom thermophilic Anoxybacillus kestanbolensis AC26Sari strain:Bioconversation of L-arabinose to L-ribulose

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Title Statement Cloning, expression and characterization of L-arabinose isomerisefrom thermophilic Anoxybacillus kestanbolensis AC26Sari strain:Bioconversation of L-arabinose to L-ribulose
 
Added Entry - Uncontrolled Name Ozer, Aysegul
Sal, Fulya Ay
Dalkıran, Nazan
Belduz, Ali Osman
Canakci, Sabriye
 
Uncontrolled Index Term Biocatalysis, Microbial pentose phosphate pathway
 
Summary, etc. L-Arabinose isomerase (L-AI) is a pivotal enzyme in the microbial pentose phosphate pathway. It is considered as asignificant biological catalyst in rare sugar production. This enzyme can isomerize L-arabinose into L-ribulose and alsoD-galactose into D-tagatose. Here, we cloned the araA gene encoding L-arabinose isomerase from Anoxybacilluskestanbolensis AC26Sari strain, sequenced and over-expressed in E. coli BL21 (DE3): pLysS. This gene is involved inL-arabinose operon in A. kestanbolensis AC26Sari. DNA sequence analysis revealed an open reading frame of 1,506 bp,capable of encoding a polypeptide of 502 amino acid residues with calculated molecular weight of 55.6776 kDa. Therecombinant was purified by heat treatment and Ni-HisTaq chromatography. The purified enzyme showed maximal activityat pH 8.5 and 65ºC and required divalent cations such as Co2+ and Mn2+ for its activity and thermostability. The apparent Kmvalue of the enzyme for L-arabinose was 6.5 mM (Vmax, 140.1002 U/mg) as determined in the precence of both 1 mM Co2+and Mn2+.
 
Publication, Distribution, Etc. Indian Journal of Experimental Biology (IJEB)
2022-05-04 17:03:29
 
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http://op.niscair.res.in/index.php/IJEB/article/view/62923
 
Data Source Entry Indian Journal of Experimental Biology (IJEB); ##issue.vol## 60, ##issue.no## 05 (2022): IJEB [May 2022]
 
Language Note en