Protective effect of eugenol from Mesua ferrea on the oxidative damages caused by 5-fluorouracil in PBMC cells
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Title |
Protective effect of eugenol from Mesua ferrea on the oxidative damages caused by 5-fluorouracil in PBMC cells
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Creator |
Surendhira, Sathees
Arjun, Mani Murugan Steven, Benhur Rajalakshmi, Perumal Vadivel, Vellingiri |
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Subject |
5-Fluorouracil
Cytoprotective effect Eugenol Mesua ferrea ROS toxicity |
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Description |
78-85
5-Fluorouracil (5-FU), a pyrimidine analogue with fluorine at C5 position, is one of the chemotherapy drugs used to treat various cancers. Though 5-FU is a very promising anticancer drug, it can cause side effects such as coronary thrombosis, intestinal mucositis, cognitive impairment, and anaemia through the generation of free radicals, damages the normal cells and also leads to apoptosis. To overcome such toxic effects of 5-FU, in the present work we have explored the cytoprotective potential of eugenol, the antioxidant active compound available in ethanolic extract of an Indian herbal drug Mesua ferrea. Aim of the present study was to evaluate the cytoprotective effect of eugenol against 5-FU toxicity in peripheral blood mononuclear cells (PBMC). Based on MTT assay, it is noticed that eugenol significantly prevents the toxicity of 5-FU (73%) in PBMC cells. Eugenol has lowered the generation of intra-cellular ROS (20035.5 AU) when compared to 5-FU treated cells (58918.5 AU). Eugenol exhibited remarkable inhibition of lipid peroxidation (43%) and cell membrane damage (LDH release: 488.02 IU/L). Thus, eugenol prevents apoptosis-induced cell death when compared with 5-FU in PBMC cells and hence it can be used to overcome the cytotoxicity caused by 5-FU and it warrants further study. |
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Date |
2022-05-12T08:54:04Z
2022-05-12T08:54:04Z 2022-03 |
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Type |
Article
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Identifier |
0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscair.res.in/handle/123456789/59693 |
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Language |
en
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Relation |
Int. cl. (2021.01)-A61K 36/00, A61P
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Publisher |
NIScPR-CSIR, India
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Source |
IJNPR Vol.13(1) [March 2022]
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