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<p style="text-align: justify;">Isolation and extraction of antimicrobial peptides from <em>Streptomyces minutiscleroticus</em> and <em>Streptomyces albogriseolus</em> from Mangrove soil of Mangalore Coast, Karnataka</p>

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Title Statement <p style="text-align: justify;">Isolation and extraction of antimicrobial peptides from <em>Streptomyces minutiscleroticus</em> and <em>Streptomyces albogriseolus</em> from Mangrove soil of Mangalore Coast, Karnataka</p>
 
Added Entry - Uncontrolled Name Jayendra, Akshatha S; Department of Studies and Research in Microbiology, Mangalore University, Jnana Kaveri Chikka Aluvara, Kodagu 571232, Karnataka, India
Kalyani, Manjula Ishwara; Department of Studies and Research in Microbiology, Mangalore University, Jnana Kaveri Chikka Aluvara, Kodagu 571232, Karnataka, India
Hosamani, Yamanappa ; Department of Studies and Research in Microbiology, Mangalore University, Jnana Kaveri Chikka Aluvara, Kodagu 571232, Karnataka, India
Science And Engineering Research Board, DST-Govt.of India , Vision Group of Science and Technology, Govt. of Karnataka
 
Uncontrolled Index Term Microbial technology, bioactive molecules, microbial based therapeutics, antimicrobial resistance, peptides/protein characterization
Antimicrobial peptides; Mangrove soil; Peptides; Phylogenetic analysis; SEM analysis; Streptomyces albogriseolus; Streptomyces minutiscleroticus.
 
Summary, etc. <p style="text-align: justify;"><em>Streptomyces </em>species are the dominant source of microorganisms involved in the production of broad-spectrum antibiotics used by many pharmaceutical and biotechnological sectors. A mangrove ecosystem with a saline and humid atmosphere harbours specific organisms that could produce significant bioactive agents that are applicable for many biomedical applications. The present study aimed to isolate, screen, with characterization and production of antimicrobial peptides from <em>Streptomyces </em>spp.<em> </em>from mangrove soil sediments. Mangrove soils were collected from four different sites on the Mangalore coast. The soil was pre-treated for the enrichment of actinomycetes and cultured with the use of five different media which exhibited distinctive morphological, biochemical, and phylogenetic features along with distinctive spore structure and arrangements by SEM image analysis. This led us to the identification of the two <em>Streptomyces </em>species, <em>Streptomyces minutiscleroticus</em> and <em>Streptomyces albogriseolus. </em>The antimicrobial screening was carried out to study their antagonistic property against the test pathogenic bacteria cultures of ATCC. In an attempt to explore peptide molecules with antimicrobial properties from actinomycetes cultures dwelling in the mangrove soil of Mangalore coast, we could enhance the peptide production in ISP2 medium for peptide production by <em>S. minutiscleroticus</em> and <em>S. albogriseolus. </em>Further, the minimum concentration of 0.01 mg microbial peptide was determined to inhibit the growth of pathogenic bacteria as assayed by broth dilution and plate assays. The LC-MS analysis revealed molecular weights of 25 kDa and 29 kDa antimicrobial peptides from <em>S. albogriseolus</em> and 31 kDa antimicrobial peptides were detected from <em>S. minutiscleroticus</em>.</p>
 
Publication, Distribution, Etc. Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]
2022-05-23 16:40:13
 
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http://op.niscair.res.in/index.php/IJNPR/article/view/52210
 
Data Source Entry Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 13, ##issue.no## 1 (2022): MARCH 2022
 
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