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<p>Flavonoid Composition and Antioxidant Efficacy of Citrus Peels: An Integrated <em>in vitro</em> and <em>in silico </em>Approach toward Potential Neuroprotective Agents</p>

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Title Statement <p>Flavonoid Composition and Antioxidant Efficacy of Citrus Peels: An Integrated <em>in vitro</em> and <em>in silico </em>Approach toward Potential Neuroprotective Agents</p>
 
Added Entry - Uncontrolled Name Sharma, Pallavi ; CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061, Himachal Pradesh, India
Dadwal, Vikas ; CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061, Himachal Pradesh, India
Rahmatkar, Shubham Nilkanth; CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061, Himachal Pradesh, India
Gupta, Mahesh ; CSIR-IHBT, Palampur, India
Singh, Damanpreet ; CSIR-Institute of Himalayan Bioresource Technology, Palampur 176 061, Himachal Pradesh, India
CSIR, India
 
Uncontrolled Index Term Citrus reticulata c.v., Hesperidin, Oxidative stress, Prediction of activity spectra, Reactive oxygen species
 
Summary, etc. <p>The current study explored the therapeutic significance of different standardized citrus peel extracts as potential neuroprotective agents using integrated <em>in vitro</em> and <em>in silico</em> approaches. Hydroethanolic extract of five industrially important citrus fruit peels were subjected to HPLC-based quantification of estimating major flavonoids of nutraceutical importance. Pharmacological activities like antioxidant and anti-inflammatory activities of the extracts were determined by <em>in vitro</em> assays. Further, The identified bioactive metabolites were subjected to the Prediction of Activity Spectra for Substances program to get a prediction of their biological activity spectrum. Amongst various solvent combinations, 80% ethanol provided maximum (≥ 20% w/w) extract yield. Mandarin peels of <em>Citrus reticulata</em> showed the highest amount of polyphenolics (<em>Citrus reticulata </em>Blanco; 42.24 ± 0.57 mg gallic acid equivalent/g) and flavonoids (<em>Citrus reticulata c.v</em>.;13.08 ± 0.17 mg quercetin equivalent/g) content. The most abundant flavonoid compound present in all the citrus peel was hesperidin, except <em>Citrus reticulata </em>Blanco and <em>Citrus grandis</em>, which showed a considerably high amount of nobiletin and naringin, respectively. <em>Citrus reticulate c.v. </em>peel extract showed potent antioxidant [IC<sub>50</sub><sub> </sub>= 118.82 ± 1.97 µg/mL in 2,2-diphenyl-1-picrylhydrazyl assay and IC<sub>50 </sub>= 138.12 ± 2.67 µg/mL in 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid assays)], anti-inflammatory (IC<sub>50 </sub>= 50.61 ± 6.79 µg/mL), and acetylcholinesterase inhibitory ((IC<sub>50 </sub>= 130.61 ± 2.04µg/mL) activities compared to the other extracts.<em> In silico</em> assessment revealed a high (Pa &gt; 0.7) activity score for free radical scavenging, lipid peroxidase inhibitory, membrane integrity agonistic, anti-inflammatory, antioxidant, and several other important biological activities of the identified flavonoids in the extracts, thus supported neuroprotective potential. Citrus flavonoids naringin, rutin, and tangeretin showed high activity scores for anti-inflammatory activity strengthening the results of <em>in vitro</em> assay. These potentials of citrus peels could be utilized in the development of functional foods and nutraceuticals for neurodegenerative conditions. Furthermore, such a practice will help citrus agro-industrial waste valorisation.</p>
 
Publication, Distribution, Etc. Journal of Scientific and Industrial Research (JSIR)
2022-06-01 22:30:46
 
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http://op.niscair.res.in/index.php/JSIR/article/view/56093
 
Data Source Entry Journal of Scientific and Industrial Research (JSIR); ##issue.vol## 81, ##issue.no## 05 (2022): Journal of Scientific and Industrial Research
 
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Nonspecific Relationship Entry http://op.niscair.res.in/index.php/JSIR/article/download/56093/465582359