Record Details

A hemagglutinin from Pongamia pinnata (L.) Pierre seed

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title A hemagglutinin from Pongamia pinnata (L.) Pierre seed
 
Creator Ayesha, Noor
Savithri, HS
Rao, AG Appu
 
Subject Drug delivery
Indian Beech tree
Karanja
Lectin
Lectin-carbohydrate interactions
Hemagglutination
Isothermal calorimetry
Millettia pinnata
Nitrogen fixing tree
Pongame oil tree
Pongamia seed lectin
 
Description 397-403
Lectins are diverse proteins that bind to carbohydrates and are involved in many important physiological processes. They
are highly specific to the sugar molecules they bind and therefore, have many therapeutic and diagnostic applications. There
are several lectins that display antiviral, antibacterial antifungal and antitumour activities. Characterization of new lectins
paves way for comprehension of their diverse biological roles and mechanism of action, thus aiding in further exploration of
lectins in various domains of biology. Here, we endeavoured to purify and characterize lectin from the seed of the
legume,Pongamia. Pongamia pinnata (L.) Pierre (syn. Millettia pinnata), seed lectin (PPSL) was purified conventionally by
ammonium-sulfate precipitation followed by size exclusion chromatography. The further lectin was physicochemically
characterized by CD, fluorescence spectroscopy, mass spectroscopy and isothermal calorimetry. Hemagglutination studies
with various mono and disaccharides showed specificity towards galactose. This specificity was reaffirmed by isothermal
studies with appreciable thermodynamic parameters. Lectins have tremendous diagnostic applications. They are used as
second-generation drug delivery systems.
 
Date 2022-06-03T06:48:19Z
2022-06-03T06:48:19Z
2022-07
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/59846
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.60(06) [June 2022]