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Regulation Of Cell Signaling By Inter-Organeller Interaction In Neuronal And Non-Neuronal Cells: Effect On MicroRNA Mediated Gene Regulation

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Field Value
 
Title Regulation Of Cell Signaling By Inter-Organeller
Interaction In Neuronal And Non-Neuronal Cells:
Effect On MicroRNA Mediated Gene Regulation
 
Creator DE, DIPAYAN
 
Subject Molecular & Human Genetics
 
Description Uponexposuretoamyloid-β oligomers(Aβ1–42), glialcellsstart
expressingproinflammatorycytokines,despiteanincreaseinlevels
of repressivemicroRNAs(miRNAs).Exploringthemechanismof
thispotentialimmunityoftargetcytokinemRNAsagainstrepressive
miRNAsinamyloid-β-exposedglialcells,wehaveidentifieddifferential
compartmentalizationofrepressivemiRNAsinglialcellsthatexplains
thisaberrantmiRNAfunction.InAβ1–42-treatedcells,whereastarget
mRNAswerefoundtobeassociatedwithpolysomesattachedto
endoplasmicreticulum(ER),themiRNAribonucleoproteincomplexes
(miRNPs)werefoundtobepresentpredominantlywithendosomes
thatfailedtorecycletoER-attachedpolysomes,preventingrepression
of mRNAtargets.Aβ1–42 oligomers,bymaskingRab7aproteinson
endosomalsurfaces,affectedRab7ainteractionwithRab-interacting
lysosomalprotein(RILP),restrictingthelysosomaltargetingand
recyclingofmiRNPs.RNA-processingbody(P-body)localizationof
themiRNPswasfoundtobeenhancedinamyloid-β-treatedcellsas
a consequenceofenhancedendosomalretentionofmiRNPs.
Interestingly,depletionofP-bodycomponentspartlyrescuedthe
miRNAfunctioninglialcellsexposedtoamyloid-β andrestrictedthe
excesscytokineexpression.
 
Date 2021
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2829/1/Thesis_for_Evaluation_DIPAYAN_DE.pdf
DE, DIPAYAN (2021) Regulation Of Cell Signaling By Inter-Organeller Interaction In Neuronal And Non-Neuronal Cells: Effect On MicroRNA Mediated Gene Regulation. PhD thesis, UNIVERSITY OF CALCUTTA.
 
Relation http://www.eprints.iicb.res.in/2829/