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Multiple shoot regeneration from excised embryonic axis explants of jute, Corchorus capsularis cv. JRC321

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Title Multiple shoot regeneration from excised embryonic axis explants of jute, Corchorus capsularis cv. JRC321
 
Creator Joshi, Alpana
Das, Subrata K.
Das, Dipak
 
Subject Corchorus sp
multiple shoot regeneration
excised embryo axis
shoot bud formation
 
Description 201-206
Interspecific hybrids development by traditional breeding is challenging because of the incompatibility between two
cultivated species of genus Corchorus. Under this condition, plant genetic transformation is an alternative tool for the
improvement of jute cultivar. In the present study, in vitro multiple shoot regeneration protocol has been developed in jute
(Corchorus capsularis cv. JRC 321) from excised embryonic axis as an explants by removing cotyledons, radicle, and tip of
epicotyl. The explant is cultured in medium shoot multiplication medium1 (SMM1) supplemented with BAP (1.0 mg/L) for
one week and sub-cultured in SMM6 containing IBA (0.5 mg/L) and BAP (0.2 mg/L). Direct adventitious shoot bud
formation was observed from cotyledonary nodes and the adjacent region of excised embryonic axis. Interestingly, shoot
bud formation was reduced when excised explants with intact epicotyl were cultured in the media combination
(SMM1+SMM6) indicating the presence of apical dominance might be the cause of fewer shoot bud formation. While,
hypocotyl explants did not produce any shoot in this media combination because nodal meristem was removed. However,
the shoot buds developed from the excised embryonic axis were differentiated successfully into multiple shoots and showed
further elongation in SMM6 which ultimately rooted in MS-B5 medium (+ 0.5 mg/L IBA). The plants were transferred
successfully in the glasshouse conditions and appeared phenotypically normal.
 
Date 2022-06-07T09:32:02Z
2022-06-07T09:32:02Z
2021-04
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscpr.res.in/handle/123456789/59862
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBT Vol.20(2) [April 2021]