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Identification and characterization of Tobacco streak virus, as a new causative agent of necrosis disease of Anthurium

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Title Identification and characterization of Tobacco streak virus, as a new causative agent of necrosis disease of Anthurium
 
Creator S, Rajamanickam
P, Renukadevi
V, Devappa
S, Nakkeeran
 
Subject Anthurium
Tobacco streak virus
coat protein gene
phylogenetic analysis
 
Description 194-200
Tobacco streak virus (TSV) was detected from Anthurium samples collected from Anthurium variety Medioria Red from
College of Horticulture, Bangalore, India grown under polyhouse cultivation. Sap inoculation of the samples expressing
positive reaction through direct antigen coating-enzyme linked immunosorbant assay (DAC-ELISA) produced both
localized and systemic infection. The localized infection produced circular necrotic lesions on the leaves of cowpea cv.
C152 plants. The samples were also confirmed through dot blot immunibinding assay (DIBA), which showed positive
reaction by producing circular, purple coloured spot against TSV specific antibody. Further molecular characterization of
TSV through RT-PCR with coat protein gene specific primers produced amplicon size of 929 bp pertaining to RNA3 region.
The basic local alignment search tool (BLAST) analysis of coat protein gene through National Centre for Biotechnology
Information (NCBI) database had nucleotide and amino acid sequence similarity of 97.6 to 98.8 % and 94.9 to 97.4 %,
respectively with known strains of TSV from India. Phylogenetic analysis of the nucleotide sequences Confirmed the
Anthurium strain of TSV forms single subgroup with other crop of Indian isolates. This study provides important clues about
spread of TSV. To the best of our knowledge it is the first detailed study of TSV infecting Anthurium.
 
Date 2022-06-07T09:44:44Z
2022-06-07T09:45:47Z
2022-06-07T09:44:44Z
2022-06-07T09:45:47Z
2022-04
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscpr.res.in/handle/123456789/59863
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBT Vol.20(2) [April 2021]