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Effects of target gene siRNA soaking in cereal cyst nematode, Heterodera avenae infecting wheat

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Title Effects of target gene siRNA soaking in cereal cyst nematode, Heterodera avenae infecting wheat
 
Creator Gantasala, Nagavara Prasad
Rao, Uma
 
Subject Quantitative real time PCR (RT-qPCR)
poly(A)-binding protein
nuclear hormone receptor-91
intron binding protein
epsin-1
 
Description 182-193
Heterodera avenae is an obligate sedentary endoparasite that causes significant crop losses in wheat, barley and oats.
Functional genomics of H. avenae is required to identify and target the essential genes involved in their pathogenesis.
Based on the available transcriptome, we have cloned and sequenced four genes (epn-1, pab-1, emb-4 and nhr-91)
differentially expressed between pre-parasitic juveniles (J2s) and feeding females of H. avenae. Further, gene expression
was evaluated in three developmental stages of H. avenae. It was noted that expression of nhr-91 and epn-1 was highest
in the feeding females while that of pab-1 was in J2s. On the other hand, expression of emb-4 was highest in the egg
stage. Microscopic observations revealed that siRNA soaking of epn-1, pab-1 and emb-4 in respective siRNA resulted in
reduced nematode development by 71%, 60%, 26.5% and reproduction by 68%, 45% and 14.4%, respectively. The
siRNA soaking of nhr-91 increased the nematode development by 25% while that of reproduction by 54%. Given the
importance of these genes in the nematode life cycle, the findings are particularly noteworthy. Our study shows target
genes epn-1 and pab-1, could serve as potential targets for effectively impairing nematode reproduction and further
development when expressed via a host-induced gene silencing approach.
 
Date 2022-06-07T09:56:03Z
2022-06-07T09:56:03Z
2021-04
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscpr.res.in/handle/123456789/59864
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBT Vol.20(2) [April 2021]