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A multigene phylogeny reveals the occurrence of fourteen dominant Penicillium species in India

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Title A multigene phylogeny reveals the occurrence of fourteen dominant Penicillium species in India
 
Creator Kamil, Deeba
Debbarma, Rubin
Thokala, Prameela Devi
Tyagi, Akanksha
Toppo, R. Sudeep
Das, Amrita
Choudhary, Shiv Pratap
 
Subject β-tub gene
DNA secondary barcode
molecular identification
multigene phylogeny
 
Description 168-181
Penicillium contains 483 accepted species worldwide but the occurrence of predominant species in India is still unknown.
Therefore, 35 isolates of 15 different states of India were procured from Indian Type Culture Collection (ITCC), New Delhi. All
these isolates were made into eight species of Penicillium viz., “P. aethiopium”, “P. chrysogenum”, “P. crustosum”,
“P. mononematosum”, “P. oxalicum”, “P. polonicum”, “P. singorense”, “P. spinulosum” and one species of “Talaromyces
pinophilus” based on morphology and multigene analysis comprises ITS, cal, rpb2 and β–tub genes. Phylogenetic analyses
using these sequences through maximum parsimonious method showed β-tub gene as a potential region for DNA barcoding to
differentiate the species of Penicillium with maximum inter population diversity (2.15), coefficient of differentiation (8.54),
barcode gap (1.93) and probability of correct identification (0.081) and it can be used as secondary barcode apart from ITS as
universal primary barcode. The β-tub gene was also validated using six more species of Penicillium (P. canescens, P. daleae,
P. janthinillum, P. ochrochloron, P. simplicissimum and P. vasconiae) isolated from soils of Delhi and Meghalaya states, where
all the species showed clear differentiation from one another. In conclusion, fourteen predominant Penicillium species was
established through morpho-molecular approach and three Penicillium species (P. mononematosum, P. singorense and
P. vasconiae) were reported first time from India and β-tub sequence-based identification was the best to confirm the
morphological based identification.
 
Date 2022-06-07T10:06:51Z
2022-06-07T10:06:51Z
2021-04
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscpr.res.in/handle/123456789/59865
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBT Vol.20(2) [April 2021]