Record Details

Quantitative proteomics identified lncRNA HOTAIR regulated nucleoproteins in HeLa cells

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Quantitative proteomics identified lncRNA HOTAIR regulated nucleoproteins in HeLa cells
 
Creator Zheng, Peng
Liu, Cheng Lin Zi
Hu, Fan
Chen, Zhuo
Zhang, Tong-cun
 
Subject lncRNA
HOTAIR
quantitative proteomics
PCNA
migration
invasion
 
Description 124-137
Hox transcript antisense intergenic RNA (HOTAIR), which was called oncogenic long non-coding RNA (lncRNA) over
expressed in the vast majority of cancer types, regulated various biological processes such as cell growth, apoptosis,
proliferation and metastasis. It can regulate hundreds of genes via recruiting complexes: polycomb repressive complex 2
(PRC2) and lysine specific demethylase 1 (LSD1). At that, majority processes happened in nucleic. However, there are no
reports to comprehensively research the regulated protein of HOTAIR in nucleic, which is important for explore the mechanism
of HOTAIR functions. In this study, we employed a quantitative proteomics analysis of HOTAIR regulated nucleoproteins in
cancer cells. Bioinformatical analysis, such as PANTHER, STRING, DAVID were used to understand the function and
relationship of these nucleoproteins. In total, we found that 224 nucleoproteins were differential expressed after inhibition
HOTAIR, implying that they could be potential targets of HOTAIR. Western blotting analysis, which was used to validation of
the quantitative proteomics data, was consistent with stable isotope labelling by amino acids in cell culture (SILAC)
experiments. Bioinformatic analysis shown that major changes in nucleoproteins involved in diverse protein class, including the
nucleic acid binding and cytoskeletal proteins. Further functional studies of proliferating cell nuclear antigen (PCNA) revealed
that various phenotypic effects observed after HOTAIR inhibition, at least in part, through the regulation of PCNA expression.
By correlating the proteomic data with these functional studies, the current results provide novel insights into the mechanism
underlying HOTAIR functions in cancer cells.
 
Date 2022-06-07T11:05:18Z
2022-06-07T11:06:21Z
2022-06-07T11:05:18Z
2022-06-07T11:06:21Z
2021-04
 
Type Article
 
Identifier 0975-0967 (Online); 0972-5849 (Print)
http://nopr.niscpr.res.in/handle/123456789/59870
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBT Vol.20(2) [April 2021]