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A comparative study of in-vitro and in-silico anti-candidal activity and GC-MS profiles of snow mountain garlic vs. normal garlic

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title A comparative study of in-vitro and in-silico anti-candidal activity and GC-MS profiles of snow mountain garlic vs. normal garlic
 
Creator Kaur , Bhupinder
Kumar, Nitish
Chawla, Sonam
Sharma, Deepika
Korpole , Suresh
Sharma, Rajni
Patel, Manoj K
Chopra, Kanwaljit
Chaurasia, Om Prakash
Saxena, Shweta
 
Subject QR Microbiology
 
Description Aim: The study aimed to profile the volatile phytocomposition of snow mountain garlic (SMG) compared to normal garlic and investigate the anti-Candida efficacy against clinically relevant multi-drug resistant isolates of Candida species.

Methods and results: Herein, SMG has shown significantly superior fungicidal power at 2x-MIC dose against C. albicans and C. glabrata in killing kinetic evaluation unlike the fungistatic effect of normal garlic. GC-MS headspace-based profiling of SMG showed 5 unique volatile compounds and a 5-fold higher content of saponins than normal garlic. In an in-silico analysis, cholesta-4,6-dien-3-ol,(3-beta) was uniquely identified in SMG as a potential inhibitor with high binding affinity to the active site of exo-1,3-betaglucan synthase, an established anti-candida drug target crucial for the biofilm matrix formation, thus suggesting a plausible anti-Candida mechanism.

Conclusion: The in-vitro and in-silico studies have demonstrated the Candida-cidal and anti-biofilm activities of SMG, distinguishing it from the Candida-static efficacy of normal garlic.
 
Publisher Wiley
 
Date 2022-04-07
 
Type Article
PeerReviewed
 
Relation https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/jam.15537
http://crdd.osdd.net/open/2979/
 
Identifier Kaur , Bhupinder and Kumar, Nitish and Chawla, Sonam and Sharma, Deepika and Korpole , Suresh and Sharma, Rajni and Patel, Manoj K and Chopra, Kanwaljit and Chaurasia, Om Prakash and Saxena, Shweta (2022) A comparative study of in-vitro and in-silico anti-candidal activity and GC-MS profiles of snow mountain garlic vs. normal garlic. Journal of applied microbiology. ISSN 1364-5072