Record Details

Exploring the Distinct Distribution of Archaeal Communities in Sites Contaminated with Explosives

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Exploring the Distinct Distribution of Archaeal Communities in Sites Contaminated with Explosives
 
Creator Pal, Yash
Mayilraj, Shanmugam
Krishnamurthi, Srinivasan
 
Subject QR Microbiology
 
Description Most of the research on bioremediation and estimation of microbial diversity in waste contaminated sites is focused on the domain Bacteria, whereas details on the relevance of Archaea are still lacking. The present study examined the archaeal diversity and predicted metabolic pathways in two discrete sites (SITE1 and SITE2) contaminated with explosives (RDX and HMX) by amplicon-targeted sequencing of 16S rRNA genes. In total, 14 soil samples were processed, and 35,758 OTUs were observed, among which 981 OTUs were classified as Archaea, representing ~2.7% of the total microbial diversity in our samples. The majority of OTUs belonged to phyla Euryarchaeota (~49%), Crenarchaeota (~24%), and Thaumarchaeota (~23%), while the remaining (~4%) OTUs were affiliated to Candidatus Parvarchaeota, Candidatus Aenigmarchaeota, and Candidatus Diapherotrites. The comparative studies between explosives contaminated and agricultural soil samples (with no history of explosives contamination) displayed significant differences between the compositions of the archaeal communities. Further, the metabolic pathways pertaining to xenobiotic degradation were presumably more abundant in the contaminated sites. Our data provide a first comprehensive report of archaeal communities in explosives contaminated sites and their putative degradation role in such ecosystems which have been as yet unexplored.
 
Publisher MDPI
 
Date 2022-03-23
 
Type Article
PeerReviewed
 
Relation https://www.mdpi.com/2218-273X/12/4/489
http://crdd.osdd.net/open/2987/
 
Identifier Pal, Yash and Mayilraj, Shanmugam and Krishnamurthi, Srinivasan (2022) Exploring the Distinct Distribution of Archaeal Communities in Sites Contaminated with Explosives. Biomolecules , 12 (4).